千金益康片对去卵巢模型大鼠E_2、FSH及相关神经递质的影响

Effects of Qianjin Yikang tablets on E_2,FSH,and related neurotransmitters in ovariectomized rats

目的:观察千金益康片对去卵巢模型大鼠血清雌二醇(E2)、卵泡刺激素(FSH)及相关神经递质的影响。方法:将60只生育龄雌性未孕SD大鼠随机分为空白对照组,模型对照组,千金益康片低、中、高剂量组和阳性药组,每组各10只,除空白对照组外,其余5组均切除卵巢;千金益康片低、中、高剂量组分别给予千金益康片混悬液5.3、10.6、21.18g/kg体质量灌胃,阳性药组给予女珍颗粒混悬液0.75g/kg体质量灌胃,空白对照组和模型对照组用等体积蒸馏水(10mL/kg体质量)灌胃,每天1次,连续14d;末次给药24h后测定各组大鼠E2、FSH水平,检测下丘脑神经递质5-羟色胺(5-HT)、去甲肾上腺素(NE)含量,计算子宫、肾上腺脏器系数。结果:与模型对照组比较,千金益康片中、高剂量组均能明显增加去卵巢模型大鼠血清E2水平(P<0.05,P<0.01);千金益康片低、中、高剂量组能明显降低去卵巢模型大鼠血清FSH水平(P<0.05);低、中、高剂量组对下丘脑5-HT含量有降低趋势(P<0.05);中剂量组能明显增加NE含量(P<0.01),低、中、高剂量组能增加子宫系数(P<0.05),而中、高剂量组能明显增加肾上腺系数(P<0.05)。结论:千金益康片可增加去卵巢模型大鼠血清E2水平,降低血清FSH水平,增加NE含量,增加子宫系数和肾上腺系数,对5-HT有降低趋势,其作用机制可能与调节内分泌系统和神经系统有关。

Objective：To observe the effects of Qianjin Yikang tablets on estradiol（ E2 ）, follicle -stimulating hormone （FSH）, and related neurotransmitters in ovariectomized rats. Methods ： Sixty non - pregnant female Sprague - Dawley rats of reproductive age were randomly and equally divided into blank control group, model control group, low -, middle, and high -dose Qianjin Yikang tablet groups, and positive medicine group. The rats in model control group, Qianjin Yikang tablet groups, and positive medicine group were ovariectomized. The low - , middle - , and high - dose Qianjin Yikang tablet groups were given Qianjin Yikang tablets （5.3,10. 6, and 21.18 g/kg, respectively） by gavage. The positive medicine group received suspension of Nuzhen granules （0. 75 g/kg） by gavage. The blank control group and model control group received equal volumes of distilled water（ 10 ml/kg）. Medication was given once daily for 14 consecutive days. At 24 h after the last administration, serum E2 and FSH levels were measured, norepinephrine （NE） and 5 hydroxytryptamine（ 5 -HT） in the hypothalamus were quantified, and the organ coefficients of uterus and adrenal gland were calculated. Results ： Compared with the model control group, the middle - and high - dose Qianjin Yikang tablet groups had significantly increased serum E2 levels（P 〈0. 05 ,P 〈0. 01 ） ;the low - , middle - , and high -dose Qianjin Yikang tablet groups had significantly decreased serum FSH levels and showed a decreasing trend in 5 - HT content in the hypothalamus （ P 〈 0. 05 ） ; the middle - dose Qian jin Yikang tablet groups had significantly increased NE content （P 〈 0. 01 ） ;low -, middle - , and high - dose Qianjin Yikang tablet groups had increased organ coefficient of uterus, and the middle - , high -dose Qianjin Yikang tablet groups had significantly increased organ coefficient of adrenal gland. Conclusion：Qianjin Yikang tablets can decrease serum FSH level and increase serum E2 level, NE content, organ coefficient of uterus and organ coefficient of adrenal gland and tend to decrease 5 - HT content. Its action mechanism may be related to its regulatory effects on the endocrine system and nervous system.