子宫内膜癌代谢综合征病理状态及微RNA-205表达分析

The pathological state of metabolic syndrome and the expressions analysis of microRNA-205 in women with endometrial cancer

目的分析子宫内膜癌手术患者代谢综合征病理状态及子宫内膜癌组织微RNA．205表达情况。方法收集38例子宫内膜癌患者腰围、体重、血压等一般资料，采集术前空腹血、术后内膜病灶及癌旁内膜组织石蜡切片。对患者代谢综合征病理状态指标进行分析，提取内膜组织总RNA，检测微RNA．205含量。结果子宫内膜癌患者代谢综合征患病率44．74％（17／38），高密度脂蛋白胆固醇、血压、三酰甘油、腰围、空腹血糖的异常率分别为52．6％（20／38）、36．8％（14／38）、34．2％（13／38）、34．2％（13／38）和26．3％（10／38）。子宫内膜癌组织中微RNA-205的表达量明显高于正常内膜组织，差异有统计学意义（P〈0．01）。结论子宫内膜癌与代谢综合征及其组分关系密切；微RNA-205参与了子宫内膜癌的发生、发展过程，有可能作为早期筛查的分子标记物。

Objective To detect the pathological state of metabolic syndrome and the expression of microRNA-205 in women with endometrial cancer. Methods Thirty-eight cases of patients with endometrial cancer were collected including waist circumference, weight, blood pressure. Collected preoperative fasting blood, postoperative endometrial lesions and endometrium tissue adjacent to carcinoma paraffin section. Analyzed the metabolic syndrome in patients with pathological state indicators,extracted the total RNA of endometrium tissue and the level of microRNA- 205 was detected. Results The prevalence of metabolic syndrome was 44.74% （17/38）. The abnormal rate of high-density lipoprotein cholesterol, blood pressure, triglycerides, waist circumference, fasting blood sugar successively were 52.6% （20/3B）, 36.8% （14/38）, 34.2% （13/38）, 34.2% （13/38） and 26.3% （ 10/38 ）. The expression level of microRNA-205 in endometrial carcinoma was higher than that in normal endometrium tissues,there was significant difference （P〈 0.01 ）. Conclusions Metabolic syndrome is closely associated with the metabolic syndrome and its components. The expressions of microRNA -205 may participate in the occurrence and development of endometrial carcinoma, and it may be as an early screening of molecular markers.