J亚群禽白血病病毒双抗体夹心ELISA检测方法的建立

Development of a Double-antibody Sandwich ELISA for Detection of Subgroup J Avian Leukosis Virus

本研究利用J亚群禽白血病病毒(subgroup J avian leukosis virus,ALV-J)gp85单因子血清纯化后的抗体成功建立了检测ALV-J抗原的双抗体夹心ELISA方法(DAS-ELISA)。结果表明,该方法具有良好的特异性、重复性和稳定性,对ALV-J抗原的最小检出量为0.165μg/mL。用该法对48份临床血浆样品进行检测,结果与PCR方法的符合率达到85.2%。

Abstract： A double-antibody sandwich ELISA （DAS-ELISA） was developed for the detection of subgroup J avian leukosis virus （ALV-J）. Antibodies of DAS-ELISA were purified from the ALV-J gp85 mono-specific serum prepared by our laboratory earlier. Results of statistics analyses showed that DAS-ELISA had good specificity, repeatability and stability. The limitation of viral antigens for detection was 0. 165 μgg/mL. The coincidence between PCR and DAS-ELISA established here was 85.2%.