不同浓度秋水仙素和nocodazole对绒山羊成纤维细胞周期同步化的影响

The Effect of Colchicine and Nocodazole in Different Concentrations on Cycle Synchronization of Fibroblast in Cashmere Goat

本试验旨在探索更加优化的条件,使绒山羊成纤维细胞经处理后更多的处于有丝分裂G2/M期。本研究分别采用秋水仙素和nocodazole试剂对绒山羊成纤维细胞进行周期同步化处理,用流式细胞仪检测G2/M期的细胞数量并进行比对,观测最佳作用浓度。结果表明,秋水仙素的使用浓度不宜过大,且添加秋水仙素时间的不同也会对试验结果产生明显的影响。另外在相同条件下,经nocodazole处理的绒山羊成纤维细胞周期同步化效果比秋水仙素好,最优的处理条件是300 ng/mL nocodazole处理成纤维细胞24 h,所得G2/M期的细胞所占比例为15.10%。结果提示,对于绒山羊的成纤维细胞来说,细胞周期同步化处理时选择nocodazole更好一些。

This experiment was conducted to explore optimized conditions to make more Cashmere goat fibroblasts which had been treated in G2/M. In the experiment, the colchicine and nocodazole reagents were used in the processing of Cashmere goat fibroblast cell cycle synchronization, and the number of the cell in G2/M was measured by flow cytometry to observe the optimal concentration and action time. The results showed that the colchicine concentration should not be too large;furthermore, the time should not be too long in treatment of colchicines. In addition, under the same conditions, the treatment of nocodazole worked better than colchicine in synchronization. The optimum processing conditions included the concentration was 300 ng/mL and the action time was 24 h. Then the percentage of fibroblast in G2/M accounted for 15.10%. The results suggested that the effect of nocodazole reagents was more pronounced for Cashmere goats fibroblasts, when conducting the cell cycle synchronization processing.