从黄芩毛状根中提取、纯化黄芩苷的研究

Study on Extraction and Purification of Baicalin from Hairy Roots of Scutellaria baicalensis Georgi

本试验以黄芩苷质量浓度为指标,考察了影响大孔吸附树脂对黄芩毛状根中黄芩苷富集效果的相关因素,并利用硅胶柱层析对其进行纯化。结果显示,LX-68型大孔吸附树脂的富集效果最好,较优工艺条件为:上样浓度为8.69 g/L,吸附流速为6 BV/h,上样体积为16 BV,树脂床径高比为1∶14;洗脱剂为70%乙醇,洗脱流速为6 BV/h,用量为10 BV;硅胶柱层析洗脱剂条件为氯仿∶乙酸乙酯∶甲醇∶甲酸(7∶3∶1.5∶0.5)。在所确定的工艺条件下,该树脂可有效地提取黄芩毛状根中的黄芩苷,黄芩苷吸附量达131.2 mg/g,黄芩苷的回收率超过69.1%,经纯化后黄芩苷纯度为98.45%。

Using the content of Baicalin as the standard, the optimum macroporous resin was selected, and the factors of Baicalin＇s extraction and purification were investigated. LX-68 macroporous resin was the suitable one, Baicalin was extracted under the optimum conditions of a sample concentration of 8.69 g/L, a adsorption speed of 6 BV/h, a sample volume of 16 BV, a diameter-high ratio of 1：14, a elution reagent of 70% alcohol, a elution speed of 6 BV/h, a elution volume of 10 BV, and then purificated by silica gel column chromatography, the condition was chloroform-ethyl acetate-methanol-formic acid（7：3：1.5：0.5）. The LX-68 macroporous resin could be used to extract Baicalin from the hairy roots of Scutellaria baicalensis Georgi, the adsorption capacity of total flavone was 131.2 mg/g, the returns-ratio was above 69.1%, and the purity of Baicalin was 98.45%.