金荞麦超微粉对小鼠免疫功能的影响

Study on the Effect of Fagopyrum dibotorys Superfines Powder on Immune Function of Mice

本试验旨在研究金荞麦超微粉对小鼠免疫功能和生长发育的影响。将60只昆明小鼠随机等分为4组,高、中、低剂量组分别灌胃1.75、1.25、0.75 g/kg体重金荞麦超微粉悬液,连续给药7 d;空白对照组灌胃等量的生理盐水。最后一次给药后间隔12 h,各组小鼠用鸡新城疫弱毒疫苗接种,随后在给药结束后的第7、14和21天取小鼠外周血用于分离淋巴细胞和血清,用CCK-8法测定外周血淋巴细胞增殖能力,微量血凝抑制试验评价血清中新城疫抗体水平,同时称量各组小鼠体重;给药结束后21 d测定小鼠腹腔巨噬细胞吞噬能力及脾脏指数。统计学分析结果显示,与对照组相比,中剂量金荞麦超微粉可极显著提高小鼠外周血淋巴细胞增殖能力、免疫抗体水平和体重(P<0.01),同时极显著提高腹腔巨噬细胞的吞噬能力和脾脏指数(P<0.01)。试验结果表明,金荞麦超微粉能显著提高机体的特异性免疫和非特异性免疫功能,促进机体的生长发育,为开发金荞麦超微粉成为新型天然植物药、饲料添加剂提供依据。

To study the effect of Fagopyrum dibotrys superfines powder on immune function and weight of mice,60 Kunming mice were divided into 4 groups,high, middle and low dose groups of mice were gavaged with the drug at a dose of 1.75, 1.25 and 0.75 g/（kg·BW） once a day for 7 consecutive days, repectively, the mice of control group were administered with the same volume of saline. 12 h after the last once, mice were injected into the muscle with Newcastle disease vaccine,then we got the peripheral blood on day 7, 14 and 21 after the medicine administrate. The enhancement of innate immune responses was evaluated by using of CCK-8 method, hemagglutination and hemagglutination inhibition test, macrophage phagocytic function test and organ coefficient method, and record the data of weight. In middle dose Fagopyrum dibotrys superfines powder group, the level of lymphocytes proliferative capacity, serum antibody, peritoneal macrophage phagocytosis and the index of immune organs were extremly significantly enhanced（P〈0.01）, and the weight was also extremly significantly increased（P〈0.01）. Fagopyrum dibotrys superfines powder could significantly regulate the immunity function and improve the weight in mice.