黑曲霉与里氏木霉固态混合发酵产β-葡萄糖苷酶

Production of β-glycosidase by co-culturing Aspergillus NL02 and Trichoderma reesei RUT-C30 through solid-state fermentation

对黑曲霉NL02与里氏木霉RUT-C30固态混合发酵产β-葡萄糖苷酶的发酵培养基进行优化,研究培养基含水率、C源、N源、接种量、温度和2种菌种不同延长接种时间与接种比例对β-葡萄糖苷酶活力的影响。研究表明:麸皮17.5 g、玉米芯7.5 g、(NH4)2SO40.40 g、尿素0.37 g、黑曲霉孢子接入量为107个接种到250 mL三角瓶中,温度30℃、摇床转速100 r/min时,里氏木霉以105个孢子与黑曲霉同时接入,每克干曲所得β-葡萄糖苷酶的活力为132.45 IU,较黑曲霉单独培养时的104.35 IU提高了26.94%。

AspergiUus NL02 and Trichoderma reesei RUT-C30 were co-cultured in solid-state fermentation to produceβ-glycosidase. To obtain the optimal enzymatic production conditions, factors including medium moisture content, carbon source, nitrogen source, quantity, temperature, strains, time, and vaccination proportion were investigated. The results showed that the optimum culture conditions were as follows：bran 17.5 g, corn cob 7.5 g, ammonium sulfate 0.40 g, urea 0.37 g, the activation spores access quantity 107spores, working in 250 mL shake flask, 30℃, 100 r/min at shaker, Trichoderma reesei with 105 spores and Aspergillus at the same time the proportion of access. Under these culture conditions, 132.45 IU/g dry medium β-glycosidase was obtained, which was much higher than 104. 35 IU/g dry medium obtained in culture with Aspergillus alone by 26.94%.