发酵法生产丁二酸的化学合成培养基的筛选

Screening of chemically defined medium for succinic acid fermentation

以产琥珀酸放线杆菌Actinobacillus succinogenes NJ113为出发菌株,针对该菌株筛选出含有关键生长因子的化学合成培养基,其关键因子为谷氨酸(Glu)、蛋氨酸(Met)和生物素(V H)和烟酸(V PP)。结合原发酵培养基中的磷酸缓冲盐成分,最终得到的化学合成培养基配方(g/L):CH3COONa 1.36,NaCl 1.0,MgCl20.2,CaCl20.2,Na2HPO40.31,NaH2PO41.6,KH2PO43,NH4HCO31.57,Glu 0.87,Met 0.11,V H0.010,V PP0.025。在3 L发酵罐上进行验证实验,50 g/L初始葡萄糖发酵70 h,丁二酸的质量浓度为45.2 g/L,丁二酸收率达到90.4%。与之前的半合成培养基发酵制备丁二酸相比,丁二酸的收率提高了25.2%,副产物也有很大幅度的减少。

According to the growth characteristics of Actinobacillus succinogenes NJll3, a chemically defined medium （CDM） was designed, which can maintain the strain to growth on. The key factors in the CDM were Glu, Met, Vn and Vpp. With phosphate buffer salts in the initial medium, the final compositions of the CDM were as follows （g/L） ： CH3 COONa 1.36, NaCl 1.0, MgC12 0.2, CaCl2 0. 2, Na2HPO4 0.31 ,NaH2PO4 1.6, KH2PO43 ,NH4HCO3 1.57 ,Glu 0.87 ,Met 0. 11 ,Vn 0. 010,Vpp 0. 025. Anaerobic batch fermentation was carried out in a 3-L fermentor in the CDM ：The initial concentration of glucose was 50 g/L, with a succinic acid concentration of 45.2 g/L and a yield of 90.4% after 70 h. Compared with the initial medium, the yield of succinic acid was increased by 25.2%, and the byproduct was remarkably decreased.