半仿生-酶法提取甘草中甘草酸的工艺研究

Semi-bionic Enzyme Extraction Method of Glycyrrhizic Acid in Glycyrrhizae Radix et Rhizoma

目的研究半仿生-酶法提取甘草中甘草酸的最佳工艺。方法分别采用-葡聚糖酶,果胶酶,提取用酶,纤维素酶以及半仿生-纤维素酶对甘草进行提取,通过高效液相色谱法检测甘草酸得率。以甘草酸得率和干膏收率为考察指标,对半仿生酶法进行正交试验优化。结果该法的最佳提取条件为提取温度80℃,料液比1:18,提取时间为2h。结论半仿生-纤维素酶提取甘草酸的方法经济有效,可作为甘草酸提取工艺应用。

OBJECTIVE To study the suitable semi-bionic enzyme extraction method of glycyrrhizic acid in Glycyrrhizae Radix et Rhizoma. METHODS fl-Dextranase, pectinase, extraction enzyme and cellulase were tested to determine which one was the most appropriate enzyme for glycyrrhizic acid extraction. The cellulase was selected and the semi-bionic cellulase extractions were performed in orthogonal experiment with three levels for three factors. Content of the glycyrrhizic acid in all the extracts were tested with HPLC. RESULTS Based on the content of glycyrrhizic acid and the extract yield, the preferred process of the orthogonal experiment was determined as： extraction temperature of 80℃, extraction time of 2.0 h and solidliquid ratio of 1 ; 18. CONCLUSION acid, which can be used in the extraction Semi-bionic enzyme extraction is an economical and effective method for glycyrrhizic process of Glycyrrhizae Radix et Rhizoma.