Box-Behnken设计-效应面法优化降糖1号胶囊的提取工艺

Optimization of Extractive Technology for Jiangtangyihao Capsule by Box-Behnken Design-Response Surface Methodology

目的优选降糖1号胶囊的提取工艺。方法水回流提取,以提取时间、提取次数、料液比为考察因素,总黄酮得率为考察指标,采用Box-Behnken设计试验,效应面法分析试验结果。结果提取时间和提取次数对实验结果具有极显著的影响,料液比对试验结果具有显著性影响,最佳提取工艺为水回流提取3次,每次59 min,料液比为23 mL·g-1,实测总黄酮得率与预测总黄酮得率的相对偏差为-2.66%。结论 Box-Behnken设计-效应面法对该提取工艺的优化结果准确、合理、可行。

OBJECTIVE To optimize the extractive technology of Jiangtangyihao capsule. METHODS Water reflux, extraction time, extraction times and solid-liquid ratio were observed as factors, total flavonoids was investigated as the indicator Box-Behnken design-response surface methodology was used to analyze test data. RESULTS Extraction time and the number of extraction times had a very significant impact on the experimental results and the solid-liquid ratio had a significant impact on the test results. The optimum extraction conditions for Jiangtangyihao capsule were obtained as follows： extracting three times with water reflux extraction, each time was 59 min, the ratio of solid-liquid was 23 mL-g-1. Relative deviation between the measured yield of flavonoids and predicted total yield of flavonoids was -2.66%. CONCLUSION The extraction method was accurate, rational and feasible.