抱石莲生物检定的方法学考察

Method Validation on the Bioassay of Lepidogrammitis Drymoglossoides(Bak.) Ching

目的 建立中药抱石莲的生物检定法。方法采用水提、醇提、水提醇沉等方法提取抱石莲有效成分，体外抑茵试验检测抱石莲中药提取物的抗菌活性及适用菌株和剂量范围，探索药物对敏感菌株的量效曲线，构建适用于一剂量法的效应模式。结果抱石莲药材的水提物具有较好的抑菌活性，其对金黄色葡萄球菌（CMCC26003）的抑菌圈直径达到17．39mm；当抱石莲的浓度为0．25-1．50g．mL-1时，抱石莲的剂量对数和反应效应呈线性关系。结论抱石莲生物检定法简单、合理、可行，可作为抱石莲质量控制的方法之一。

OBJECTIVE To establish a bioassay method for Lepidogrammitis drymoglossoides（Bak.） Ching. METHODS Three methods including water extraction, ethanol extraction, and water extraction plus ethanol subsiding method were used to extract the bioactive components from Lepidogrammitis drymoglossoides（Bak.） Ching, and the anti-bacterial effect of each extraction was detected by the anti-bacterial test in vitro. The standard bacterium strain and the dose range of the medicine were selected. We ploted the curve of dose-effect ofLepidogrammitis drymoglossoides（Bak.） Ching in response to sensitive bacterium strains and established an effective model applied to one-point method. RESULTS The water extraction of Lepidogrammitis drymoglossoides（Bak.） Ching showed the diameter of its inhibition zone to CMCC 26003 strain was 17.39 mm. There was a good linear correlation between logarithm dosages and effect of Lepidogrammitis drymoglossoides（Bak.） Ching when its concentrations ranged from 0.25 to 1.25 g.mL-1. CONCLUSION The bioassay method is one of the quality control methods of Lepidogrammitis drymoglossoides（Bak.） Ching, which is simple, reasonable and feasible.