丹皮酚对高血脂模型大鼠肠系膜上动脉的舒张作用

Vasodilatation Effect of Paeonol on Superior Mesenteric Artery of Hyperlipemia Rats

目的研究丹皮酚对健康和高血脂模型大鼠肠系膜上动脉的舒张作用。方法高脂乳剂灌胃法制备大鼠高血脂模型。以离体微血管张力描记技术记录健康大鼠和高血脂模型大鼠肠系膜上动脉的张力。结果丹皮酚可浓度依赖性地舒张重酒石酸去甲肾上腺素(NA)、5-羟色胺(5-HT)及内皮素(ET-1)等预收缩的健康大鼠肠系膜上动脉最大舒张分别达到NA、5-HT及ET-1预收缩高度的(98.86±0.31)%、(99.77±0.32)%和(99.27±0.18)%,与正常对照组比较,差异均有统计学意义(P<0.01)。此外,丹皮酚可浓度依赖性地舒张氯化钾(KCl)和5-HT预收缩的高血脂模型大鼠肠系膜上动脉,最大舒张分别达到KCl和5-HT预收缩高度的(98.98±0.72)%和(98.96±0.64)%,与正常对照组比较,差异均有统计学意义(P<0.01)。结论丹皮酚对健康和高血脂模型大鼠的肠系膜上动脉均有显著舒张作用,有望成为治疗缺血性血管疾病的有效药物。

Objective To investigate the vasodilative effect of paeonol on superior mesenteric artery in healthy and hyperlipemia rat. Methods Hyperlipemia model was established by intragastric administration of high-fat emulsion. Angiotasis of mescnteric arteries was recorded by a myograph system in vitro. Results Paeonol relaxed the superior mesenterie artery of healthy rat precontracted by norepinephrine （NA）, 5- serotonin receptor（5-HT） and endothelin （ET-1）, and the maximum relaxation was （98.86±0.31）% , （99.77 ±0.32）% and （99.27 ±0.18）% of the precontraction respectively（P 〈 0.01 compared with the control）. And also, Paeonol relaxed the superior mesenteric artery or hyperlipemia rat precontracted by potassium chloride （KCI） and 5-HT, the maximum relaxation was （98.98 _＋0.72）% and （98.96 _＋0.64）% of the precontraction respectively （P 〈 0.01 compared with the control）. Conclusion Paeonol induces vasodilatation on mesenteric artery in both healthy and hyperlipemia rat. Therefore, paeonol can be expected as an effective drug effect on ischemic diseases of blood vessels.