耳叶牛皮消和戟叶牛皮消的谱学对比分析

Spectrophotometry and Chromatography Analysis of Cynanchum bungei and Cynanchum auriculatum

目的研究耳叶牛皮消和戟叶牛皮消二者谱学性质上的差异。方法综合运用紫外-可见光谱(UV-Vis)、红外光谱(FT-IR)和高效液相色谱法(HPLC)研究耳叶牛皮消和戟叶牛皮消在石油醚、氯仿、乙酸乙酯、甲醇和水5种不同提取溶剂中的谱学行为,宏观比较谱图中峰的数目、形状和强度,以及4种主要活性物质的相对含量。结果在紫外光谱对比分析中,耳叶牛皮消和戟叶牛皮消的石油醚和水提取部位有较大差别。在红外光谱对比分析中,耳叶牛皮消和戟叶牛皮消的石油醚、氯仿和水提取部位波谱形状差别较大。在液相色谱对比分析中,二者所含主要成分的种类基本相同,但是,不同类成分的含量有较大差异。结论确定了耳叶牛皮消和戟叶牛皮消谱学性质的差别,为耳叶牛皮消和戟叶牛皮消的化学手段鉴别提供了理论依据和技术支持。

Objective To investigate the differences of characteristic spectrum of Cynanchum auriculatum Royle ex Wight and Cynanchum b ungei Decne. Methods Ultraviolet-visible spectrum （UV-Vis）, Fourier transform infrared spectroscopy （FT-IR） and high performance liquid chromatography （HPLC） were applied to investigate the spectral behavior such as peak amount, peak shape and peak intensity as well as relative content of four major bioactive substances in five different extracted parts of C. auriculatum and C. btmgei. Results The results of UV-Vis analysis showed that petroleum ether extract and water extract of the two kinds of medicinal material showed significant difference. FT-IR analysis indicated that great differences of peak shape were shown among the petroleum ether extract, chloroform extract and water extract of the two kinds of medicinal material. HPLC results indicated that the main substances in C. auriculatum and C. bungei were basically the same, but the variation of the contents between them was great. Conclusion Spectral differences in C. auriculatum and C. bungei have been demonstrated, and the results will provide the theoretical basis and technical support for the chemical identification of C. auriculatum and C. b unge i,