补肾益肺消癥方干预肺纤维化大鼠TGFβ信号通路的作用机制

Functional Mechanism of prescription of nourishing lung and kidney and elimination on the intervention of signal pathway of TGF in pulmonary fibrosis rats

目的:探讨补肾益肺消癥方治疗特发性肺纤维化疗效的作用机制。方法:以博莱霉素致大鼠肺纤维化为动物模型,补肾益肺消癥方进行干预;检测血清中TGF-b1含量的差异、肺组织中TGF-b1R基因表达的改变以及Smad2/3、Smad4蛋白含量的差异。结果:该方可以明显下调IPF大鼠外周血血清中TGF-b1的含量和Smad2、3的表达,对TGF-bR1基因表达无影响。结论:通过调控TGF-b信号传导通路,缓解炎症反应并抑制肺组织纤维化,是补肾益肺消癥方疗效的关键作用机制之一。

Objective： To explore the mechanism of Bushenyifeixiaozheng Formula in the treatment of idiopathic pulmonary fibrosis （IPF）. Methods ：The IPF rats were caused by Bleomycin and treated by Bushenyifeixiaozheng Formula. The contents of transforming growth factor （TGF）- bl in serum of rats were detected by enzyme linked immunosorbcnt assay （ELISA）. The gene expression of TGF-bl receptor （TGF-blR） in lung of IPF rats was detected by real-time PCR. The protein contents of Smad2/3 and Smad4 were detected by western-blot. Results： TGF-blcontent in the serum of IPF rats was down regulated through treated by this formula, but the gene expression of TGF-bl R was no change. Smad2and Smad3 of the key molecules in TG-F-b signal pathway could be down regulated by this formula. Conclusion： This formula should interfere in the TGF-b signal pathway, release the inflammatory reaction and inhibit the process of pulmonary fibrosis. This should be the key mechanism of IPF treated by this formula.