摘要
目的观察针刺对胚胎着床障碍大鼠子宫内膜趋化因子受体CCR2表达的影响。方法将72只孕鼠随机分为正常组(N)、胚胎着床障碍组(M)、针刺治疗组(A)和黄体酮治疗组(W),每组随机再分为第6天组(D6)、第8天组(D8)、第10天组(D10)。其中M组、A组和W组大鼠妊娠第1天给予米非司酮麻油溶液造模,N组仅给予等量麻油溶液。同时,A组固定并每天针刺三阴交、后三里,N组和M组仅固定,W组肌肉注射黄体酮,直至处死。用免疫组织化学法、Western blot和Real-time PCR等方法测定着床点子宫内膜CCR2的表达。结果与N组相比,M组大鼠子宫内膜CCR2蛋白和mRNA的表达均明显降低,差异有统计学意义(P<0.05);与M组相比,A组和W组大鼠子宫内膜CCR2蛋白和mRNA的表达则明显增高(P<0.05)。结论针刺能上调胚胎着床障碍大鼠母胎界面CCR2的表达,从而促进胚胎的着床和胎盘的形成。
Objective To observe effect of acupuncture on expression of CCR2 in endometrium of rat with em-bryo implantation dysfunction.Methods 72 pregnant rats were randomly divided into normal group (N ) ,model group(M) ,acupuncture treatment group(A) ,and progestin treatment group(W).Then the rats in each group were equally randomized into D6 group ,D8 group ,and D10 group.The rats in the M ,A and W groups were treated with mifepristone-sesame oil solution on the first day ,while the rats in N group were injected with the same amount of sesame oil.'Housanli' and 'Sanyinjiao' were selected for acupuncture.The rats in A group were fasten up and then performed acupuncture from the first day to the time of death.Accordingly ,the rats in the N and M groups were only fixed ,while the rats in W group were given progestin.The expression of CCR2 in rat endometrium was detected by immunohistochemistry ,western blot and real-time PCR.Results Compared to N group ,the protein and mRNA expression of CCR2 were significantly decreased (P〈 0.05);Compared to group M ,the protein and mRNA expression of CCR2 were increased obviously in rat endometrium of A group and W group (P〈 0.05) . Conclusion Acupuncture can up-regulate the level of CCR2 at maternal-fetal interface of rats with embryo implan-tation dysfunction ,and thus play an important role in embryo implantation and placentation.
出处
《中西医结合研究》
2013年第4期176-180,共5页
Research of Integrated Traditional Chinese and Western Medicine
基金
国家自然科学基金项目(No.30973833)
关键词
针刺
胚胎着床障碍
子宫内膜
CCR2
acupuncture
embryo implantation dysfunction
endometrium
CCR2