一种简单高效的水稻体细胞诱变育种新方法

A New and Effective Somatic Mutagenesis Rice Breeding Method

多样化种质资源的利用对于培育高产和理想农艺性状水稻品种显得非常重要.在本研究中,通过研究继代时间、2,4-D浓度和不同外植体对诱变和分化频率的影响,建立了幼穗为外植体、继代培养时间为3个周期(约75d)以及2,4-D的最佳诱变浓度为4.0mg/L的体细胞诱变育种方法.然后利用该方法诱变杂交水稻亲本株1S和中9B,分别成功选育了优良矮化突变株系SV14S和SV9B,为后续的杂交育种提供了优质种质资源.这些育种成果的取得进一步证实了该水稻体细胞诱变育种方法是一种简单高效的新型育种方法.

The availability of diversified germplasm resources is most important for developing im- proved rice varieties with higher seed yield and other desired agronomic traits. In this study, the effect of subculture time, 2,4-Dichlorophenoxyacetic acid （2,4-D） concentration and variant explants on the fre- quencies of plant regeneration and mutagenesis were investigated, and a new somatic mutagenesis breeding method for rice with the immature inflorescences as explants, 3 cycles of subculture time （about 75 d） and 4.0 mg/L 2,4-D as the preferred mutagenic concentration was developed. Then, the parents of hybrid rice, ZhulS and Zhong 9B, were mutagenized in this new method, and 2 elite semi-dwarf somatic lines, SV14S and SV9B, were successfully isolated, which will provide high-quality germplasm resources for fol-low-up cross-breeding. The breeding achievements made in this study further confirm that this rice somatic mutagenesis breeding method is a simple and effective breeding method.