铜绿假单胞菌制剂经由甘露糖依赖方式影响人宫颈癌Hela细胞株的生长研究

PA-MSHA Inhibits Growth of Human Cervical Cancer Cell Line Hela via a Mannose- dependent Manner

目的研究铜绿假单胞菌制剂(pseudomonas aeruginosa with mannose sensitive hemagglutination pili,PAMSHA)由甘露糖依赖方式影响Bad、Bax及Bcl-2的表达进而影响癌细胞的增殖和细胞周期进程,为治疗宫颈癌的新的生物制剂提供依据。方法采用单独处理PA-MSHA或甘露糖与PA-MSHA联合处理宫颈癌Hela细胞,随后采用MTT法检测细胞增殖,流式检测细胞周期,荧光定量PCR和western blot检测凋亡抑制蛋白Bcl-2和促凋亡蛋白Bad、Bax的表达变化。结果 PA-MSHA单独处理宫颈癌Hela后细胞增殖减慢,细胞周期阻滞在G0/G1期,同时荧光定量PCR和western blot检测显示促凋亡蛋白Bad、Bax的表达增加而凋亡抑制蛋白Bcl-2表达减少。而甘露糖与PA-MSHA共培育组与空白组对比在细胞增殖、细胞周期及凋亡蛋白表达上差异无统计学意义。结论 PA-MSHA可以影响Bad、Bax及Bcl-2的表达进而影响癌细胞的增殖和细胞周期进程,且这种作用为甘露糖敏感型,该结果为其成为治疗宫颈癌的新的生物制剂提供了可靠依据。

Objective To investigate the effects of Pseudomonas aeruginosa with mannose sensitive hemagglutinin （PA- MSHA） on the proliferation and cell cycle of cervical cancer cell line Hela via a mannose- dependent manner. Methods Hela cells were treated with PA- MSHA or PA MSHA and mannose. The cell proliferation was observed by MTT. Cell cycle distri- bution was measured by flow cytometry （FCM）. Real time RT-PCR and western blot were used to evaluate the expressions of apoptosis - related molecules, Bad, Bax and Bcl - 2. Results For the Hela cells treated with PA- MSHA alone, the cell pro- liferation slowed down. GO - G1 cell cycle arrest was observed. The expressions of Bad and Bax were strongly increased while that of Bcl - 2 was deceased. For the Hela cells co- cultured with PA MSHA and mannose, the cell proliferation, cell cycle and the expression of the apoptcxsis- related proteins were not significantly different from those of the controls（ P 〉 0.05 ）. Conclu- sions PA- MSHA can affect the expression of Bad, Bax and Bcl - 2 and further influcence the proliferation and cell cycle of the cancer cells. The effects are manncse - sensitive. The data provide mechanistic details for the potential application of PA- MSHA based treatment against human cervical cancer.