副流感嗜血杆菌鉴定中三种不同方法对Ⅴ因子需求试验比较分析

Comparative Study of Three Methods of V Factor Requirement Test for the Identification of Haemophilus parainfluenzae

目的了解副流感嗜血杆菌鉴定中Ⅴ因子需求性试验与不同平板琼脂卫星试验的比较分析。方法采用嗜血杆菌分离培养基(HAE)分离细菌,在MH平板及营养琼脂平板上对分离的细菌进行Ⅴ因子需求性试验。结果Ⅴ因子需求性试验结果表明,营养琼脂点种法与营养琼脂纸片法结果差异无统计学意义,但MH琼脂平板点种法与营养琼脂纸片法差异较明显。结论用营养琼脂平板点种法进行副流感嗜血杆菌Ⅴ因子需求试验结果与纸片法相符,MH平板点种法阳性结果明显偏低,造成副流感嗜血杆菌漏检,流感嗜血杆菌假阳性增高。

Objective To compare V factor requirement test and satellite test on different agar media for the identifica- tion of Haemophilus parainfluenzae. Methods Haernophilus was cultured in the simple selective culture medium. MH agar medium and nutrient agar medium were used for V factor requirement test. Results The results of V factor requirement test by dibbling method on nutrient agar medium had no statistical significance with those by paper disk method on nutrient agar me- dium, but there were statistically significant differences between dibbling method on MH agar medium and paper disk method on nutrient agar medium. Conclusions V factor requirement test of Haemophilus by dibbling method on nutrient agar medium was consistent with that by paper disk method. The positive rate of dibbling method on MH agar is significantly low, which caus- es the possible undetectable result of Haemophilus parainfluenzae and the higher false positive rate of Haemophilus influenzae.