^(60)Co γ辐射对毛竹种子萌发过程中内源激素含量的影响

Effect of ^(60)Co γ Rays Radiation on Endogenous Hormone During Seed Germination

为了研究不同剂量的60Coγ辐射对毛竹种子萌发过程中内源激素含量的影响,使用高效液相色谱法检测种子内源ABA、GA3、IAA、ZR的含量及其比值变化。结果表明15 Gy和30 Gy辐射处理后,与对照相比提前12 h萌发,加快了种子萌发进程,GA3/ABA、IAA/ABA、ZR/ABA和(GA3+ZR)/ABA均高于对照,有利于种子打破休眠。辐射剂量为60 Gy、90 Gy、120 Gy时,GA3、IAA、ZR含量降低,ABA含量与对照相比显著增多,GA3/ABA、IAA/ABA、ZR/ABA和(GA3+ZR)/ABA值与对照相比较小,抑制萌发。由此可见,低剂量(15~30 Gy)的60Coγ射线辐射促进可以促进种子萌发进程,较高剂量(60~120 Gy)的60Coγ射线辐射可以抑制种子的萌发进程。

The aim was to study the effects of the endogenous hormones contents during Pbyllostacchys edulis seed germination, which were irradiated by different does of ^60Co γ rays. The endogenous hormones of ABA, GA3, IAA, ZR were examined by high-performance liquid chromatography. The results showed that the germination process could be accelerated by 15 Gy ^60Co γ and 30 Gy ^60Co γ rays, thereby led to increased values of GA3/ABA, IAA/ABA, （GA3＋ZR）/ABA and ZR/ABA, consequently promoted seed germination 12 h compared with the control. 60-120 Gy ^60Co γ rays could inhibit the germination process, the content of GAg, IAA, ZR were decreased, while ABA was increased, the values of GA3/ABA, IAA/ABA, （GA3＋ZR）/ABA and ZR/ABA were decreased compared with CK. Hence, low doses of ^60Co γ rays （15-30 Gy） could promote seedlings, higher doses of ^60Co γ rays （60-120 Gy） could inhibit seedlings.