深绿木霉生物学特性研究

Biological Characteristics of Trichoderma Strain

从灰树花染病时分离出1株具生防潜力的木霉菌,为了明确其生物学特性,对该菌株进行了形态学鉴定,并对其生长培养基、温度、pH、碳源、氮源和维生素等生物学特性进行了研究。结果表明：该菌为深绿木霉;该菌株在PDA、MEA、基本培养基和查彼培养基上均能生长,但在PDA培养基上生长最快,2天即能长满平皿。在10~37℃范围内,菌丝均能生长,以25℃时菌丝生长最快,30℃时产孢量最大。在pH 2~10范围内,菌丝菌均能生长,pH 5~7时菌丝生长最快。牛肉膏为菌丝生长的最佳氮源,葡萄糖为最佳的碳源,维生素B6对菌丝生长最有利。

Trichoderma is a potential biocontrol fungus which was isolated from Gritlola frondosa, the morphology and their biological characteristics of Trichoderma were investigated. The results showed that, it could grow on the PDA, MEA, basic and Czapek culture medium, but PDA was the most favorable for the hyphae growth, where the plant could be covered with culture dish in 2 days. From 10% to 37% it could grow and the optimal temperature for the growth was 25℃, while 30% was best for the sporulation. It could grow on the media with a wide range of pH value from 2 to 10, and the optimum pH value was 5 to 7. Beef extract was the optimum nitrogen source and glucose was the best carbon source; VB6 was the best vitamin for hyphae zrowth.