生物活性追踪法对丁香抗低密度脂蛋白氧化修饰的研究

Oxidation Modification of Clove Anti-low-density-lipoprotein by Bio-assay Guided Method

丁香为桃金娘科植物丁香的干燥花蕾。实验室前期经筛选发现,丁香在国家公布的87种药食两用物品原料中抗氧化活性最强。为了在此基础上进一步探索丁香抗低密度脂蛋白(low density lipoprotein,LDL)氧化修饰作用,本文采用生物活性追踪及硫酸铜诱导法,对丁香抗LDL氧化修饰作用进行了研究。结果表明,反应体系中硫酸铜和LDL浓度分别采用3 mmol/L和4 mg/mL合适。丁香粗提物及其乙酸乙酯相、正丁醇相与水相三个极性部位对LDL氧化均具有相应的抑制能力。在不同极性部位中乙酸乙酯相抑制率最高(85.29±0.02)%,为有效部位。该有效部位对LDL抑制呈现良好的量效关系,且对已被氧化修饰的LDL也存在抑制能力。薄层色谱分析发现有效部位主要由8种成分组成。实验证明丁香具有很强的LDL氧化修饰抑制能力,为后续研发抗动脉粥样硬化功能食品打下了基础。

Clove, the dried buds ofEugenia caryophylata Thunb, showed the strongest antioxidant activity among 87 kinds of medicinal and edible plants in our previous researches. In this research, the anti-Low-Density-Lipoprotein （LDL） oxidation modification of clove was studied by detemaining the yield of Malondialdehyde and using bio-assay guided method and copper sulfate induction. The results showed that the appropriate concentrations of copper sulfate and LDL were 3 mmol/L and 4 mg/mL, respectively. The crude extract and different fractions of clove possessed strong anti-LDL oxidation activity. The inhibition efficiency of ethyl acetate fraction was higher （85.29±0.02）% than that extracted by ethyl acetate n-butyl alcohol and water. The inhibition rote of ethyl acetate fi＇action on LDL oxidation modification presented a good dose-effect relationship. There are eight kinds of ingredients in effective fraction through TLC analysis.