黄缘盒龟多肽对D-半乳糖致亚急性衰老小鼠抗氧化能力的影响

Effect of Yellow-margined Box Turtle Muscle Polypeptides on Antioxidant Activities in D-galactose-induced Aging Mice

为研究黄缘盒龟多肽对D-半乳糖致亚急性衰老小鼠抗氧化能力的影响,本试验采用连续皮下注射D-半乳糖(200mg/(kg·d))建立小鼠亚急性衰老模型,造模6周。建模同时灌胃给予黄缘盒龟多肽50、100、200 mg/(kg·d),以肌肽(50 mg/(kg·d))为阳性对照物质。6周后对小鼠血清、肝组织和脑组织中丙二醛(MDA)含量、总抗氧化能力(T-AOC)水平、超氧化物歧化酶(SOD)活力和谷胱甘肽过氧化物酶(GSH-Px)活力进行测定。研究表明,黄缘盒龟多肽的抗氧化效果与黄缘盒龟多肽的给药剂量呈正相关关系。与模型对照组相比,黄缘盒龟多肽能极显著提高血清中SOD活力41.71%和降低血清中MDA含量28.80%(p<0.01);也能显著提高血清中T-AOC水平31.08%、脑中SOD活力22.08%及血清和肝组织中GSH-Px活力22.94%和14.53%(p<0.05);同时使肝组织和脑组织中MDA含量显著降低22.03%和11.88%(p<0.05)。因此,黄缘盒龟多肽可以提高D-半乳糖致亚急性衰老模型小鼠的抗氧化能力,具明显的抗衰老作用。

The antioxidant activities in vivo of yellow-margined box turtle muscle polypeptides were investigated by orally feeding D-galactose-induced aging mice. A subacute aging mice model was established by subcutaneous injection of D-galactose （200 mg/（kg.d）） for 6 weeks. Using L-camosine as positive control, yellow-margined box turtle muscle polypeptides were taken to mice by gavage at three different doses of 50, 100 and 200 mg/（kg-d）. The mice were slaughtered after 6 weeks, and the activities of total antioxidant capacity （T-AOC）, serum superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and the content of malondialdehyde （MDA） in the serum, liver and brain were assayed. The results showed that the antioxidant effects of yellow-margined box turtle muscle polypeptides were positively correlated with the increased dosages. Compared with model control group, yellow-margined box turtle muscle polypeptides could significantly increase SOD activity by 41.71% and reduce MDA level by 28.80% in the serum （p 〈 0.01）. An obvious increase of T-AOC activity by 31.08% in the serum and SOD activity by 22.08% in the brain was also observed （p 〈 0.05）. Meanwhile, the activity of GSH-Px in the serum and liver exhibited an obvious increase by 22.94% and 14.53% （p 〈 0.05）. Furthermore, the content of MDA in the brain and liver revealed a reducing trend by 22.03% and 11.88% （p 〈 0.05）, respectively. Therefore, the yellow-margined box turtle muscle polypeptides showed obvious antioxidant activities in vivo, and they would have a great prospect for deep development and comprehensive utilization.