摘要
目的:探讨多西他赛(docetaxel)和多柔比星(doxorubicin,ADM)对乳腺癌细胞迁移、侵袭能力以及丝状伪足形成的影响。方法:以三阴性乳腺癌细胞系MDA-MB-231为研究对象。实验分为3组:空白对照组、多西他赛组和ADM组,利用细胞计数试剂盒-8(cell counting kit-8,CCK-8)检测多西他赛和ADM对肿瘤细胞的IC_(10)值。利用Transwell小室法检测两种化疗药物在IC_(10)值浓度下,对乳腺癌细胞的迁移和侵袭能力的影响;在排除了此浓度对细胞增殖的影响后,用荧光素罗丹明(rhodamine)标记的鬼笔环肽着色细胞骨架,观察两种药物对肿瘤细胞丝状伪足形成的影响。结果:多西他赛和ADM对MDA-MB-231细胞的IC_(10)值分别为1.0 ng/mL和0.5μg/mL;在这两种质量浓度下,对细胞增殖活性并无影响,对细胞周期的分布也无影响(P>0.05);Transwell小室法检测结果显示,1.0 ng/mL的多西他赛较0.5μg/mL的ADM对肿瘤细胞的迁移、侵袭能力有更好的抑制作用(P<0.05);多西他赛对抑制三阴性乳腺癌细胞丝状伪足形成有更加突出的效应,两组细胞存在明显差异。结论:多西他赛较ADM能更好地抑制肿瘤细胞的迁移和侵袭,这与其抑制肿瘤细胞丝状伪足的形成可能存在相关性。
Objective：To investigate the effects of docetaxel and ADM （doxorubicin） on migration, invasion and filopodia formation of breast cancer cells. Methods： Triple-negative breast cancer MDA- MB-231 cells were divided into three groups： blank control group, docetaxel group and ADM group. CCK-8 （cell counting kit-8） assay was used to determine the IC10 （inhibitory concentration of 10%） values of docetaxel and ADM. Abilities of invasion and migration were detected by Transwell assay under IC10 of docetaxel and ADM. The effect of these concentrations on cellular proliferation was assayed by flow cytometry to detect the cell cycle. The rhodamine-phalloidin was used to stain cell cytoskeleton to observe filopodia formation after treatment with these two drugs. Results： The IC10 values of docetaxel and ADM in MDA-MB-231 cells were 1.0 ng/mL and 0.5 μg/mL, respectively. These two concentrations had no effects on cellular proliferation and cell cycle （P 〉 0.05）. Docetaxel （1.0 ng/mL） could inhibit migration and invasion of tumor cells more effectively than ADM （0.5 μg/mL）. Docetaxel inhibited filopodia formation of triple-negative breast cancer cells more effectively with a statistical significance. Conclusion： Docetaxel can obviously inhibit the migration and invasion of breast cancer cells as compared with ADM, which may be associated with suppression of filopodia formation by these agents.
出处
《肿瘤》
CAS
CSCD
北大核心
2013年第9期776-780,共5页
Tumor
