髓源性抑制细胞在胃癌中的分布及临床意义

Frequency of myeloid-derived suppressor cells in gastric cancer and its clinical signiicance

目的:检测髓源性抑制细胞(myeloid-derived suppressor cells,MDSCs)在人胃癌患者外周血及肿瘤组织中的分布比例,并分析其分布水平与胃癌临床病理因素的关系。方法:收集38例胃癌患者术前外周血及手术切除肿瘤组织,使用免疫荧光标记和FCM法分析MDSCs在外周血及肿瘤组织中所占的比例,以及MDSCs表面标志物(CD3、CD14、CD19、CD56、CD11b、CD1а及CD18)的表达情况;统计学分析MDSCs分布与临床病理因素之间的相关性。结果:MDSCs存在于胃癌患者外周血及肿瘤组织中,所占比例较正常人显著升高;其表面表达髓系抗原而不表达造血细胞标志物;MDSCs的比例与胃癌患者的幽门螺旋杆菌感染及肿瘤分期密切相关。结论:MDSCs在胃癌肿瘤微环境的扩增参与了胃癌的进展,可能是胃癌肿瘤生物治疗研究的一条新途径。

Objective： To detect the proportion of MDSCs （myeloid-derived suppressor cells） in the peripheral blood and dissected tissues of gastric cancer, and to analyze the correlation between the frequency of MDSCs and the clinical and pathological parameters. Methods： The peripheral blood samples before surgical operation and the dissected tissues of gastric cancer from 38 cases were collected. The immunofluorescence and flow cytometry were used to detect the proportions of MDSCs and the expressions of their surface antigens including CD3, CD14, CD19, CD56, CD1 lb, CDla and CD18 in the peripheral blood and the cancer tissues. The statistic analysis was used to analyze the correlation between the frequency of MDSCs and the clinical and pathological parameters. Results： MDSCs were found in peripheral blood and cancer tissues with a remarkable high proportion in patients with gastric cancer as compared with that in the healthy volunteers. The myeloid cell antigens were expressed on the surface of MDSCs but the expressions of lineage antigens were absent. The frequency of MDSCs was related to H. pylori （helicobacter pylon） infection and cancer staging. Condusion： The expansion of MDSCs is involved in development of gastric cancer and this may become a new study focus on gastric cancer intervention.