塞来昔布抑制人胃癌细胞MGC803细胞增殖及其分子机制

Modulating Effect of Celecoxib to Proliferation and Molecular Mechanism in MGC803 of Gastric Carcinoma Cell Line

目的:研究塞来昔布在体外对人胃癌细胞MGC803生长增殖及其抗肿瘤的相关分子机制。方法:体外培养人胃癌细胞MGC803,MTT法检测塞来昔布在相同浓度下,不同时间对于胃癌细胞增殖的影响,并计算IC50值;RT-PCR法检COX-2、MMP-9的mRNA的表达影响;结果:MTT结果显示:相同干预浓度塞来昔布抑制人胃癌细胞增殖,其24 h,48 h,72 h的IC50分别为:(98.67±11.755)μmol/L、(67.506±6.646)μmol/L、(57.662±15.809)μmol/L;RT-PCR结果显示:人胃癌细胞MGC803正常对照组及塞来昔布干预组COX-2mRNA灰度值分别为:0.918±0.031,0.301±0.002(t=16.037,P=0.000);MMP-9mRNA灰度值分别为:0.928±0.041,0.360±0.012(t=10.487,P=0.003);结论:塞来昔布抑制胃癌细胞增殖,塞来昔布抗肿瘤机制可能通过抑制COX-2及MMP-9的mRNA的表达来实现的。

Objective ：To study celecoxib in vitro human gastric cancer cell growth and proliferation of MGC803 and its anti-tumor molecular mechanisms. Methods Cultured human gastric cancer MGC803, MTT method analyses celecoxib at the same concentration, at different times for the gastric cancer cell proliferation and calculate IC50 value; RT-PCR method used to evaluate the expression of COX-2, MMP-9 mRNA . Results MTT assay showed： the concentration of the same intervention celecoxib to inhibit the proliferation of human gastric cancer cells, its 24h, 48h, 72h IC50 of respectively： 98.67 ± 11.755μ mol/L,67.506 ± 6.646 μmol/L,57.662 ± 15.809 μmol/L; The RT-PCR results show： human gastric cancer ceils MGC803 normal control group and celecoxib in the intervention group, respectively, the COX-2mRNA gray value for： 0.918 ± 0.031,0.301 ± 0.002 （t=16.037,P=0.000）; MMP-9 mRNA gray values were： 0.808 ± 0.021,0.101 ± 0.002 （t=19.037,P=0.000）;MMP-9 mRNA were 0.928 ± 0.041,0.360 ± 0.012 （t=10.487, P=0.003 ） ; Conclusions celecoxib inhibition of gastric cancer ceil proliferation, celecoxib antitumor mechanisms may by inhibiting the expression of COX-2 and MMP-9 mRNA in implementation.