大籽獐牙菜的化学成分研究

Chemical constituents of Swertia macrosperma

目的研究大籽獐牙菜Swertia macrosperma的化学成分。方法利用高效液相色谱、柱色谱、重结晶等方法进行分离纯化,通过理化性质和波谱数据分析鉴定化合物的结构,采用半叶枯斑法进行抗烟草花叶病毒(TMV)活性实验。结果从大籽獐牙菜全草甲醇提取物中共分离得到8个化合物,分别鉴定为9,10-二羟基獐牙菜苷(1)、3′-O-(3-羟基苯甲酸酯)獐牙菜苷(2)、芒果苷(3)、2,6,8-三羟基酮-1-O-β-葡萄糖苷(4)、1,2,3,4-四氢-1,6,8三羟基酮-4-O-葡萄糖苷(5)、1,2,3,4-四氢-1,4,6,8-四羟基酮(6)、顺-4,4′-二羟基二苯基乙烯(7)、反-4,4′-二羟基二苯基乙烯(8)。结论化合物1为新化合物,命名为大籽獐牙菜苷A,其具有微弱的抗TMV活性,其中化合物2、7、8为首次从獐牙菜属植物中分到。

Objective To study the chemical constituents from Swertia macrosperma. Methods HPLC, column chromatography, and recrystallization techniques were used for the separation and purification of the compounds. Their structures were elucidated by physiochemical properties and spectral analyses. The inhibitory activity against tobacco mosaic virus （TMV） was screened by half-leaf blight spot assay. Results Eight compounds were isolated from S. macrosperma and their structures were identified as 9, 10- dihydroxyl-sweroside （1）, 3′-O-（3-hydroxybenzoyl）-swertiamarin （2）, mangiferin （3）, 1-O-β-glucopyranosyl-2, 6, 8-trihydroxyl- xanthone （4）, campestroside （5）, bellidifolin （6）, 4, 4′-dihydroxy-Z-stilbene （7）, and 4, 4′-dihydroxy-E-stilbene （8）. Conclusion Compound 1 is a new compound named macrospermaoside A, and exhibits weak anti-TMV activity. Compounds 2, 7, and 8 are isolated from the plants of this genus for the first time.