微弧氧化钛表面对成骨细胞形态及细胞骨架的影响

Microarc oxidation of titanium surfaces on osteoblast morphology and cytoskeleton

目的研究微弧氧化钛表面对成骨细胞形态及细胞骨架的影响。方法将直径15 mm、厚度1 mm的纯钛片根据表面处理方法不同分为4组:机械打磨(G)组、喷砂(SB)组、打磨微弧氧化(GMAO)组和喷砂微弧氧化(SBMAO)组。采用扫描电子显微镜(SEM)、激光共聚焦显微镜(LSCM)研究钛片表面成骨细胞生长情况及细胞骨架的改变。结果成骨细胞接种12 h后,各组细胞均沿钛片表面铺展开,且GMAO组和SBMAO组细胞覆盖于火山口状微孔上。各组肌动蛋白纤维清晰可见,GMAO组和SBMAO组肌动蛋白纤维平行排列,汇聚成束伸向微孔内。结论微弧氧化后的钛表面可以影响成骨细胞铺展的形态及细胞骨架的排列。

Objective This study aimed to evaluate the effects of the microarc oxidation surface on cell morphology and cytoskeleton. Methods Pure titanium with a diameter of 15 mm and a thickness of 1 mm was divided into four groups： grooved surfaces （group G）; sandblasted surfaces （group SB）; grooved microarc oxidation surfaces （group GMAO）; and sandblasted microarc oxidation surfaces （group SBMAO）. Osteoblast cells were cultured in each group. The morphology and proliferation of the cells on the titanium surface were observed by scanning electron microscope （SEM）. The cytoskeleton was evaluated by laser scanning confocal microscope （LSCM）. Results The osteoblasts were inoculated after 12 h; these osteoblasts then spread along the surface of the titanium plate. In GMAO and SBMAO groups, the osteoblasts converged in the hole. The actin fibers in each group were clearly visible. In particular, the actin fibers in GMAO and SBMAO groups were arranged in parallel and formed bundles that extended into the holes. Conclusion After microarc oxidation, the tita- nium surface significantly affected the morphology and cytoskeleton of osteoblasts.