锌指蛋白217在甲状腺乳头状癌组织中的表达及意义

Expressions and significance of ZNF217 in papillary thyroid carcinoma

目的探讨锌指蛋白217（ZNF217）在人类甲状腺乳头状癌组织中的表达及其意义。方法利用实时荧光定量PCR法和Western印迹法检测20例甲状腺乳头状癌组织及相应的20例癌旁正常组织中ZNF217的mRNA和蛋白表达水平，并进行统计学分析。结果ZNF217mRNA和蛋白在甲状腺乳头状癌组织中的表达显著高于癌旁正常甲状腺组织（96．72±44．19对4．86±3．55，0．994±0．172对0．195±0．061，均P〈0．01），在甲状腺乳头状癌包膜侵犯组高于无包膜侵犯组（P〈0．01）。ZNF217mRNA和蛋白的表达与患者的性别、年龄、肿瘤大小、TNM分期及有无淋巴结转移均无关（均P〉0．05）。结论ZNF217的过表达可能与甲状腺乳头状癌的发生发展和包膜侵犯有关，有望成为甲状腺乳头状癌预防和治疗的一个新的靶点。

Objective To investigate the expression and clinical significance of zinc finger gene 217 （ZNF217） in human papillary thyroid carcinoma. Methods The expressions of ZNFE17 mRNA and protein were detected by quantitative reahime PCR and Western blot in papillary thyroid carcinoma tissues （ n = 20） and adjacent normal tissues （ n = 20 ） , and the data were analyzed. Results The expressions of ZNF217 mRNA and protein in papillary thyroid carcinoma were significantly higher than those in adjacent normal tissues （96.72 ± 44.19 vs 4.86 ±3.55,0. 994 ± 0. 172 vs 0. 195 ± 0. 061, both P〈0.01 ） , being higher in the papillary thyroid carcinoma with capsule invasion compared with that without capsule invasion （ P〈0.01 ）. The expressions of ZNF217 mRNA and protein in papillary thyroid carcinoma were not related to gender, age, tumor size, TNM stage or lymph node metastasis （ all P〉 0.05 ）. Conclusions The overexpression of ZNF217 may be associated with the oncogenesis and progress of papillary thyroid carcinoma and capsule invasion, and thus is expected to become a new target for prevention and treatment of papillary thyroid carcinoma.