GHR和IGF-IR在济宁青山羊子宫内分布和表达的发育性变化

Developmental Changes of the Distribution of GHR and IGF-IR and the Expression of Its mRNA in the Uterus of Jining Grey Goats

旨在探讨济宁青山羊生后发育过程中生长激素受体(Growth hormone,GHR)和胰岛素样生长因子I受体(Insulin-like growth factor-I,IGF-IR)在子宫的分布及其mRNA的表达规律。运用实时荧光定量PCR技术,检测GHR mRNA和IGF-IR mRNA的表达规律;SABC法检测GHR和IGF-IR免疫阳性细胞在子宫的分布。结果表明,GHR和IGF-IR免疫阳性染色主要见于子宫腺管上皮、基质、血管内皮和子宫平滑肌细胞的胞质中,偶见胞核着色。GHR和IGF-IR的阳性细胞数与其mRNA表达的变化趋势基本一致,且IGF-IR低于GHR。GHR和IGFIR在初情期(60日龄)和性成熟(120日龄)达到高峰(P<0.01),120日龄后下降,并维持在一个相对稳定的水平。GHR和IGF-IR在子宫的生长发育过程中起着重要作用,且初情期和性成熟期可能是济宁青山羊生后子宫发育最快的时期。

To study the localization of GHR and IGF-IR and the expression of GHR mRNA and IGF-IR mRNA in uterus of Jining Grey goats during postnatal development. Real-time floures- cence quantitative PCR was used to detect the expression pattern of GHR mRNA and IGF-IR mRNA, and SABC method was applied to research the distribution of positive cells of GHR and IGF-IR in the uterus. GHR and IGF-IR were mainly localized in the cytoplasm of glandular epi- thelium, stromal cells, endothelium and smooth muscle cells of the uterus, occasionally in the nuclei. The changed trends on the positive cells of GHR and IGF-IR were similar with that on the expression of its mRNA. Although the expression of IGF-IR was less than that of GHR, the ex- pression of GHR and IGF-IR both reached its peak on the puberty （D60） and the sexual maturity （D120）（P〈0.01）, Then decreased and remained at a relatively stable level. GHR and IGF-IR play an important role in the growth of the uterus, and the puberty and the sexual maturity may be the fastest period of the uterus during postnatal development.