人参皂苷Rb1对H9N2亚型猪流感病毒诱导急性肺损伤小鼠肺组织氧自由基的影响

Effect of Ginsenoside Rb1 on Oxygen Free Radicals in Acute Lung Injury of Mice Induced by H9N2 Swine Influenza Virus

本试验将120只6～8周龄BALB／c小鼠随机分为对照组、肺损伤模型组（ALI组）、人参皂苷Rb1组（G-Rb1组），每组40只。AL1与GRb1组采用100μL SIA／swine／HeBei／012／2008／猪流感病毒（H9N2SIV）经鼻腔接种建立急性肺损伤模型，同时G-Rb1组腹腔注射人参皂苷Rb1液0．1mL（剂量为10mg·kg-1），连续7d；对照组鼻腔接种相同剂量生理盐水稀释的正常鸡胚尿囊液。通过观察临床症状、小鼠的体重、肺病理组织学变化、检测小鼠肺组织T—SOD活性、抑制0H·能力及MDA、NO含量，探讨人参皂苷Rb1（G-Rb1）清除肺损伤小鼠氧自由基的能力。结果表明，从第2天末开始ALI组大部分小鼠出现高度的精神沉郁，呼吸极度困难，采食量明显减少，体重下降。肺部明显水肿、淤血和出血，炎性细胞渗出，对照组小鼠各器官未见异常。G-Rb1组症状明显轻于攻毒组，症状出现较缓，症状较轻，死亡时间延迟，死亡率降低。在第4、6、8天：抑制0H·能力、T-SOD活力与对照组比，G-Rb1与AILI组含量显著减少（P〈O．05），但G-Rb1组明显高于ALI组（P〈0．05）；MDA及N0含量与对照组比，G-Rb1与ALI组含量极显著增加（P〈0．01），但G-Rb1组明显低于ALI组（P〈0．01）。本研究表明，G-Rb1在一定浓度范围内，具有清除由病毒诱导的肺损伤组织中自由基的作用，保护自由基对肺组织的氧化损伤。

To study effect of ginsenoside Rb1 on oxygen free radicals in acute lung injury of mice induced by A/swine/HeBei/012/2008/swine influenza virus （H9N2 SIV）, 120 six to eight weeks old BALB/c mice were randomly divided into three groups with forty in each. The mice in control group were inoculated intranaslly with an equivalent dilution of noninfectious allantoic. And that of acute lung injury group （ALI group） and Ginsenoside Rb1 group （G-Rb1 group） both were in- oculated intranasally with H9N2 SIV diluted in sterile saline,and meanwhile,animals of G-Rb1 group were treated with Ginsenoside Rb1 （10 mg . kg-1） by intraperitoneal injection continuous- ly for up to seven days. The clinical signs and body losses were observed in eight infected mice of each group. At the same time,at the indicated time points after infection,lung histopathology wasobserved and the activity of T-SOD,inhibition ability of OH ·, MDA and NO content of mouse lungs were detected. The results showed that mice of ALI group appeared depression, ruffled fur, feed intake reduction and weight loss post first 2 days of infection. Furthermore, pulmonary ede- ma,hemorrhage,and a number of inflammatory cells exuding from the alveolar were observed in lungs of infected mice, however, mice organs in the control group showed no abnormality. For mice in G-Rb1 group, clinical symptoms were significantly improved, while survival time was de- layed and mortality was decreased. On the 4th, 6th and 8th day after infection.the inhibition ability of OH ~ and T-SOD activity were significantly reduced （P〈0. 05） in ALI group and G-Rb1 group mice compared with that of control group mice,but the indexes of G-Rb1 group were signif- icantly higher than that of ALI group （P〈0.05）;The contents of NO and MDA were increased significantly （P〈0.01） in ALI group and G-Rb1 group mice compared with that of control group mice, but these contents of G-Rb1 group was significantly lower than that of ALI group （P〈0.01）. Results indicate that G-Rb1 could scavenge free radicals in acute lung injury lung induced by H9N2 SIV in mice,and G-Rb1 is helpful to attenuate the lung injury induced by oxygen radi- cals.