反相高效液相色谱法测定人血浆中伊洛哌酮的含量

Determination of iloperidone in human plasma by RP-HPLC

目的建立反相高效液相色谱(RP-HPLC)法测定人体血浆中伊洛哌酮的含量。方法色谱柱为美国Diamonsil(?)C_(18)反相柱(250mm×4.6mm,5μm),流动相为乙腈-甲醇-缓冲液(40:55:5,V/V/V),流速为1.0 mL·min^(-1),柱温35℃,检测波长280 nm。结果伊洛哌酮血浆质量浓度在0.021~2.688 mg·L^(-1)范围内线性关系良好(r=0.998 9),平均相对回收率为98.21%,平均日间精密度为6.02%。结论本法快速、简便、准确,适于伊洛哌酮药动学研究和临床监测。

AIM To establish a RP-HPLC method for content determination of iloperidone in human plasma. METHODS A Diamonsil C_18 column （250 mm×4.6 ram, 5 μm） was used with mobile phase of acetonitrile- methanol-buffer （40 ： 55 ： 5, V/V/V） . Flow rate was 1.0 mL＇min-1, column temperature was 35 ℃. UV detection wavelength was 280 nm. RESULTS Within a mass concentration range of 0.021 - 2.688 mg·L-1, Standard curve of iloperidone had a good linearity （r = 0.998 9）. The average relative recovery was 98.21%, and the average daytime precision was 6.02%. CONCLUSION This method is rapid, convenient and accurate. It can be used for the pharmacokinetics study and clinical monitor of iloperidone.