白硇砂和紫硇砂不同炮制品抗炎作用及急性毒性研究

Study on anti-inflammatory effect and acute toxicity of different processed products of Sal Ammoniac and Halite Violaceous

目的探讨白硇砂和紫硇砂不同炮制品的抗炎作用和安全性。方法采用二甲苯致小鼠耳廓肿胀和小鼠腋窝皮下置无菌棉球致炎实验,均取60只小鼠随机分成6组,每组10只,为生理盐水组、地塞米松组、白硇砂醋捞品组、白硇砂醋煮品组、紫硇砂醋捞品组及紫硇砂醋煮品组。除生理盐水组外,其余按0.2 g·kg^(-1)剂量灌胃给药,分别测定小鼠耳廓肿胀度及肉芽肿重量。采用小鼠体内急性毒性实验,评价白硇砂和紫硇砂不同炮制品的急性毒性。结果紫硇砂醋捞品和醋煮品组耳廓肿胀的抑制率分别为31.7%和28.1%,棉球肉芽肿组织的抑制率分别为18.2%和19.6%,耳廓肿胀度和肉芽肿重量与生理盐水组相比均降低(P<0.05或P<0.01)。白硇砂各炮制组小鼠耳廓肿胀度及棉球肉芽肿重量与生理盐水组相比,均无显著差异(P>0.05)。小鼠口服白硇砂醋捞品和醋煮品半数致死量(LD_(50))分别为3.74 g·kg^(-1)和3.63 g·kg^(-1),口服紫硇砂醋捞品和醋煮品LD_(50)分别为5.36 g·kg^(-1)和5.29 g·kg^(-1)。结论紫硇砂不同炮制品对急性和亚急性动物炎症模型有对抗作用,白硇砂各炮制品无明显作用;白硇砂、紫硇砂不同炮制品的急性毒性差异不大,在药典推荐剂量内应用是安全的。

AIM To study the anti-inflammatory effects and safety of different processed products of Sal A mmoniac and Halite Violaceotts. METHODS The anti-inflammatory effect of different processed products was studied by mice auricular swelling test induced by xylene and mice inflammation model induced by planted cotton ball in axillary. Sixty mice were randomized into six groups （n = 10 each group）, such as sodium chloride group, dexamethasone group, Sal Ammoniac L （extracted with vinegar） group, Sal Ammoniac Z （boiled with vinegar） group, Halite Violaceous L group, and Halite Violaceous Z group. The administered dosage were 0.2 g ·kg-1 except sodimn chloride group. The mice ear edema and the weight of cotton ball granuloma were determined respectively. The safety was evaluated by acute toxicity experiment. RESULTS The products of Halite Violaceous extracted with vinegar and boiled with vinegar could inhibit the mice ear edema induced by xylene with the inhibition rates of 31.7% and 28.1% respectively, and they could also decrease the weight of cotton ball granuloma with the inhibition rates of 18.2% and 19.6% respectively, which have significant difference with sodium chloride group （P 〈 0.05 or P 〈 0.01）. However, there was no significant difference among groups of the processed products of Sal Ammoniac and sodium chloride. The oral LDso of the products of Sal Ammoniac extracted with vinegar and boiled with vinegar were 3.74 g ·kg-1 and 3.63 g· kg-1 respectively, which of Halite Violaceous were 5.36 g·kg-1 and 5.29 g·kg-1. CONCLUSION The results suggested that only the processed products of Halite Violaceous showed anti-inflammatory effects on both acute and subacute animal models. It is safe to use the processed products of Sal Ammoniac and Halite Violaceous by oral administration with normal clinical dosage.