脂肪来源干细胞对光老化成纤维细胞的治疗作用

The therapeutic effect of adipose-derive stem cells on photoaging fibroblasts

目的探讨脂肪来源干细胞（ADSCs）对光老化成纤维细胞的治疗作用。方法分离培养小鼠ADSCs，并进行表面标志物鉴定；ADSCs与成纤维细胞（FBs）共培养48h后，单次UVB120mJ／cm2照射后30min检测细胞内活性氧（ROS）生成量；采用UVB120mJ／cm2反复照射4次，照射间隔时间12h，诱导产生细胞光老化模型，继续培养48h后检测细胞增殖能力、合成弹性蛋白原等变化。结果体外培养ADSCs呈成纤维细胞样，活力良好，表面标志物CD29、CD90．2和sca-1阳性，CD34和CD45阴性；ADSCs可减轻单次UVB照射引起的氧化应激损伤，保护细胞免受细胞周期阻滞的影响，同时可促进光老化FBs合成弹性蛋白原。结论ADSCs对光老化FBs具有保护作用，可作为对抗光老化的新手段。

Objective To investigate the therapeutic effect of adipose-derived stem cells （ADSCs） on photo-aging fibroblasts （FBs）. Methods ADSCs were isolated and cultivated in vitro, identified by surface markers. After co-culture with transwell chambers for 48 hours, FBs were irradiated by a series of 4 UVB expo- sure at the dose of 120 mJ/cm2. The generation of reactive oxygen species （ROS） was detected immediately af- ter one single exposure of UVB at 120 mJ/cm2. The proliferation capacity and secretion of tropoelastin were e- valuated 48 hours after the last exposure with co-culture of ADSCs. Results ADSCs were fibroblast-like cells in culture, maintaining satisfactory cell viability. ADSCs were positive for CD29, CD90.2, Sca-1, and negative for CD34 and CD45. The generation of ROS was reduced by ADSCs co-culture. The photoaging FBs showed cell cycle arrest while ADSCs counteracted this effect. Regarding secreting function, ADSCs rescued the pho- toaging FBs by enhancing tropoelastin synthesis. Conclusion ADSCs were here demonstrated protective role on photoaging FBs, and could serve as a new potential therapy in anti-photoaging skin.