bFGF对多孔高密度聚乙烯-软骨复合支架血管化的实验研究

Experimental study of bFGF on porous high density polyethylene-cartilage scaffold vascularization

目的观察碱性成纤维细胞生长因子（bFGF）在多孔高密度聚乙烯（Medpor）-软骨复合支架血管化中的应用效果，探讨其使用意义。方法构建兔耳软骨与Medpor复合支架模型，植于兔背部皮下，将实验动物分成2组：耳软骨与Medpor材料直接连接组（A组）；耳软骨与Medpor材料直接连接后，bFGF处理组（术中及术后2d，每日局部注射bFGF100ng，B组）；置入后第3、7、14、28天后取出复合支架，行大体和组织学观察，免疫组织化学法（SABC法），检测Medpor片边缘及内部CD34表达，并行微血管密度（MVD）计数。结果B组术后早期Medpor材料周边纤维组织增生及血管化较A组明显，但后期血管化程度与A组无明显差异。结论术后早期应用bFGF对复合支架的纤维血管化过程中起到明显的促进作用，加强Medpor与软骨片的连接，增强复合支架的稳定性及抗感染力。

Objective To observe the application effect of basic fibroblast growth factor （bFGF） in the medpor-cartilage scaffold vascularization, and explore its significance. Methods Build rabbit ear cartilage with Medpor bracket model, implanted in rabbits subcutaneously, and the animals were divided into two groups ： the ear cartilage and Medpor material directly connected group （ Group A ） ; the ear cartilage directly connected with medpor, and the scaffold was pretreated with bFGF（ 100 ng/d×3 d, Group B）. At the 3, 7, 14, 28 clays after implantation, remove the composite scaffold for gross and histological observation, immuno- histochemical method （SABC method ） detection of CD34 expression in composite scaffolds and parallel mi- crovessel density （MVD） count. Results Proliferation of fibrous tissue and vascularization in the early postop- erative Group B than Group A was significantly, but no significant difference in the late vascularization with group A. Conclusion Early postoperative application of bFGF play a significant role in promoting for compos- ite scaffolds fibrovascular process, and strengthen the connection between the Medpor material and cartilage piece, enhance the stability of the composite scaffold and anti-infectious.