摘要
目的 研究纳米氧化铜对小鼠的遗传毒性及氧化应激水平.方法 30只小鼠随机分成对照组、50、150 mg/kg纳米氯化铜染毒组,染毒组分别一次性腹腔注射50、150 mg/kg纳米氧化铜,对照组给予含0.05% Tween 80相同体积的生理盐水.于染毒前及染毒后24、48及72 h分别测定尾静脉外周血网织红细胞微核发生率和尿中8-羟基-脱氧鸟嘌呤核苷(8-OH-dG)水平.染毒72 h,处死小鼠,取肝、肾和股骨骨髓,分别提取DNA并测定DNA中8-OH-dG.结果 50 mg/kg染毒组48 h,150 mg/kg组24和48 h外周血网织红细胞微核发生率(3.1 1‰±1.46、4.25‰±0.43‰、5.42‰±0.76‰)比两组染毒前(1.55‰±0.39‰、1.11‰±0.19‰)及相应的对照组(1.55‰±0.28‰、1.00±0.67‰)明显升高,差异有统计学意义(P<0.05或P<0.01).与染毒前及对照组比较,各时间50、150 mg/kg染毒组尿8-OH-dG水平(ng/mg尿肌酐)均明显升高,差异有统计学意义(P<0.05或P<0.01).50和150 mg/kg组肝组织DNA中8-OH-dG水平(4.53±1.27、7.69±2.78)明显高于对照组(0.85±0.14),差异有统计学意义(P<0.01).结论 纳米氧化铜对小鼠具有遗传毒性,并致其氧化应激水平升高.
Objective To investigate the genotoxicity and oxidative stress induced by copper oxide nanoparticles in mice.Methods Thirty mice were randomly divided into control group and low-and high-dose exposure groups.The low-and high-dose exposure groups were given copper oxide nanoparticles (50 and 150 mg/kg) by a single intraperitoneal injection,while the control group was given an equal volume of normal saline containing 0.05% Tween 80.The micronucleus rate of reticulocytes in peripheral blood from the caudal vein and urinary 8-hydroxy-deoxyguanosine (8-OH-dG) level were measured before and at 24,48,and 72 h after exposure.All the mice were sacrificed at 72 h after exposure,the liver,kidney,and femoral marrow were taken for DNA extraction,and 8-OH-dG in DNA was quantified.Results The micronucleus rates of peripheral blood reticulocytes in low-dose exposure group at 48 h (3.11±1.46‰) and in high-dose exposure group at 24 and 48 h (4.25±0.43‰ and 5.42±0.76‰) were significantly increased compared with those before exposure (1.55±0.3‰and 1.11±0.19‰) and those in control group (1.55±0.28‰ and 1.00±0.67‰) (P<0.05 or P<0.01).The urinary 8-OH-dG levels (ng/mg creatinine) in low-and high-dose exposure groups at all time points were significantly increased compared with those before exposure and those in control group (P<0.05 or P<0.01).The low-and high-dose exposure groups had significantly higher content of 8-OH-dG in liver DNA than the control group (4.53±1.27 and 7.69±2.78 vs 0.85±0.14,P<0.01).Conclusion Copper oxide nanoparticles cause genotoxicity and increase oxidative stress in mice.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2013年第9期690-692,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
日本产业医科大学博士研究生科研促进基金
关键词
纳米氧化铜
遗传毒性
氧化应激
copper oxide nanoparticles
genetoxicity
oxidative stress