β-葡聚糖对大鼠增生性玻璃体视网膜病变的作用及其机制研究

Anti-proliferative effects and mechanism of β-glucan on proliferative vitreoretinopathy in rats

目的探讨β-葡聚糖对Wistar大鼠增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)的影响。方法 52只Wistar大鼠随机分为空白对照组(A组)、模型对照组(B组)及β-葡聚糖高(C组)、中(D组)、低(E组)浓度治疗组。造模后B、C、D及E组分别向大鼠实验眼玻璃体内注入生理盐水、40 g·L-1β-葡聚糖溶液、20 g·L-1β-葡聚糖溶液及10 g·L-1β-葡聚糖溶液。各组于造模后7 d、14 d、21 d、28 d行裂隙灯及间接眼底镜检查;造模后28 d所有模型鼠取出眼球,制作标本并行组织病理学检查,采用免疫组织化学法检测视网膜组织中Smad7表达水平,应用ELISA法检测玻璃体液中转化生长因子-β1(transforming growth factor-beta 1,TGF-β1)表达水平。结果 C组术后21 d、28 d PVR分级严重程度与B组差异均有统计学意义(Z=-2.392、-2.520,均为P<0.05);D、E组术后28 d PVR分级严重程度与B组差异均有统计学意义(Z=-3.120、-2.943,均为P<0.05)。造模后28 d时B、C、D及E组的Smad7表达光密度值分别为0.108±0.013、0.598±0.015、0.358±0.017、0.349±0.023,C、D、E组与B组差异均有统计学意义(t=-6.001、-3.062、-2.952,均为P<0.05)。造模后28 d时各组玻璃体液中TGF-β1的浓度分别为(50.11±2.15)pg·mL-1、(145.12±3.08)pg·mL-1、(85.66±2.86)pg·mL-1、(109.13±2.56)pg·mL-1、(115.16±2.18)pg·mL-1,B、C、D、E组与A组差异均有统计学意义(t=-1163.630、-435.397、-722.844、-796.697,均为P<0.05),B组与C、D、E组差异均有统计学意义(t=728.233、440.786、366.934,均为P<0.05),D、E组与C组差异均有统计学意义(t=-287.448、-361.300,均为P<0.05),E组与D组差异有统计学意义(t=-73.852,P<0.05)。结论β-葡聚糖可能通过上调Smad7表达、降低TGF-β1水平途径抑制PVR发展,具有防治PVR的潜能。

Objective To investigate the effects of β-glucan on proliferative vit-reoretinopathy（PVR） in Wistar rats. Methods Fifty-two Wistar rats were ramdonfly divided into blank control group（ A）, model control group（ B）, β-glucan high concentra- tion treatment group（ C）, β-glucan medium concentration treatment group （D）, and β- glucan low concentration treatment group （E）. When the model established, rats in group B, C, D, E were injected to the vitreous with normal saline, β-glucan of 40 g · L-1 ,20 g · L-1 ,and l0 g · L-1 ,respectively. Rats of each group were examined by slit lamp and indirect ophthalmoscope on the 7th day d, 14th day,21st day and 28th day. Eyeballs of model rats were extracted to make samples, to analyze histopathologically on the 28th day, and Smad7 expression level in retinal tissues was detected immunohis- tochemically. Expression level of transforming growth factor-beta 1 （ TGF-β ） in vitre- ous was tested by ELISA. Results The difference of PVR severity between group C on the 21st day and 28th day postoperatively and group B was found statistically significant （Z = - 2. 392, - 2.520, all P 〈 0.05 ）. The differences of PVR severity among group D and E on the 21st day and 28th day postoperatively and group B were found statistically significant （ Z = - 3. 120, - 2. 943, all P 〈 0. 05 ）. On the 28th day of model establish- ment,Smad7 expression of optical density in group B, C, D, E were 0. 108 ± 0. 013, 0.598 ±0. 015,0. 358± 0.017,0. 349 ± 0. 023 ,respectively. The differences among group C,D ,E and group B were of statistical significance （ t =- 6.001, - 3. 062, - 2. 952, all P 〈0.05 ）. TGF-β1 concentration in vitreous of each group was （50. ll ± 2. 15 ）pg · mL-1,（145.12＋23.08）pg · mL-1,（85.66 ±2.86）pg · mL-1,（109. 13±2.56）pg · mL-1, （115.16 ±2.18）pg· mL-1 ,respectively on the 28th day of model establishment.The differences between group B,C,D,E and group A had statistical significance （t = - 1163. 630, -435. 397, -722. 844, - 796.697, all P 〈 0.05 ） ; the differences between group C, D, E and group B also were of statistical significance （ t = 728. 233, 440.786,366.934, all P 〈 0.05 ） ; the differences between group D, E and group C were found statistically significant （ t = - 287. 448, - 361. 300, both P 〈 0. 05 ） ; the difference between group E and group D was statistically significance （ t = - 73.852 ,P 〈 0.05 ）. Conclusion ^-glucan may inhabit PVR development through raising Smad7 expression and lowering TGF-~I level.