玉米再生相关基因ZmLEC1的序列变异及其与胚性愈伤组织形成能力的关联分析

Sequence Diversity of ZmLEC1 and Association Analysis of Embryogenic calli Formation Ability in Maize

以95份玉米微核心种质和3份常用玉米转基因受体材料(A188、HiII和综31)组成的关联作图群体,对玉米再生相关候选基因ZmLEC1进行序列变异分析,并利用候选基因关联分析策略揭示该基因与胚性愈伤组织形成能力的关系,发掘提高胚性愈伤组织形成能力的有益等位变异。结果表明,不同材料之间的幼胚胚性愈伤组织形成能力和再生能力有显著差异,其中粤267-1-1诱导的愈伤组织和国际上普遍利用的HiII极为相似,胚性愈伤组织率达到98.48%,可以用于幼胚的遗传转化。ZmLEC1基因多态性分析表明,在852 bp编码区内共发现33个SNPs和9个INDELs,LD衰减距离为300 bp(R2=0.1),ZmLEC1基因中4个多态性位点与胚性愈伤组织形成能力存在显著关联。

A maize association mapping population consisted of a mini core collection of ninety-five maize inbred lines and three elite maize accessions（A188,HiII,and Zong 31）usually for genetic transformation was used to analyze the sequence diversity and linkage disequilibrium（LD）of ZmLEC1,a candidate gene of regeneration ability in maize.A candidate gene association strategy was used to reveal the relationship between this gene and embryogenic calli formation ability and discover favorable alleles and genotypes enhancing the embryogenic calli formation ability.The results showed that there existed significant differences in abilities of embryogenic calli formation and regeneration among these accessions.The calli induced from Yue 267-1-1 were very similar to those of HiII,the popularly used genotype in maize transformation.Yue 267-1-1 had the highest ability of embryogenic calli formation and regeneration and could be a new germplasm for immature embryo-based genetic transformation.The result of sequence polymorphism analysis of ZmLEC1 showed that there were thirty-three SNPs and nine InDels in the coding region of 852 bps.The LD between all of the informative polymorphisms decayed rapidly to about 300 bp at R2=0.1.Totally four polymorphic sites in the ZmLEC1 gene were significantly associated with embryogenetic calli formation ability.