产低温脂肪酶菌株CZW001发酵培养基优化研究

Optimization of fermentation medium for low-temperature lipase-producing strain CZW001

【目的】研究产低温脂肪酶菌株CZW001发酵培养基。【方法】在单因素试验的基础上,采用Plackett-Burman(P-B)设计、Box-Behnken(B-B)设计和响应面试验设计(RSM),在20°C、pH 8.0、160 r/min发酵2 d条件下,对发酵培养基进行优化。【结果】该菌株最适产酶培养基为(g/L):葡萄糖7.68,橄榄油21.93,硫酸铵2.0,磷酸二氢钾1.0,硫酸镁0.27,氯化钙0.3,氯化钠20.0,吐温-80 1.0。其最高酶活为62.8 U/mL,比优化前提高了3.14倍。【结论】通过对产低温脂肪酶菌株CZW001发酵培养基优化研究,明显提高低温脂肪酶活力。

[Objective] Research on the fermentation medium to produce low-temperature lipase strain CZW001. [Methods] Based on single factor test results, the liquid fermentation medium for the production of cold-active lipase by CZW001 was optimized using Plackett-Burman, Box-Behnken and response surface method under follow conditions： temperature 20 °C, pH 8.0, stirred velocity 160 r/min and fermentation time 2 d. [Results] The optimal fermentation medium of the strain is as follows （g/L）： glucose 7.68, olive oil 21.93, （NH4）2SO4 2.0, KH2PO4 1.0, MgSO4·7H2O 0.27, CaCl2 0.3, NaCl 20.0, Tween-80 1.0. Under these conditions, the highest total enzyme activity （62.8 U/mL） was obtained and increased by 3.14 times compared with the original value before optimization. [Conclusion] The study on optimizing the fermentation medium to produce low-temperature lipase strain CZW001 obviously improved the lipase enzyme activity at low temperature.