不同前处理方法对陈化烟叶致香成分测定的影响

Effect of Different Preprocessing Methods on Determination of Aroma Compositions in Aging Tobacco

采用同时蒸馏萃取(SDE)、顶空固相微萃取(HS-SPME)、吹扫捕集-热脱附(P&T-TD)3种前处理方法结合气相色谱-质谱(GC-MS)联用技术分析陈化烟叶致香成分的种类和含量的差异。结果表明,吹扫捕集-热脱附法所得致香成分129种,同时蒸馏萃取法所得致香成分105种,顶空固相微萃取法所得致香成分101种;从所得烟叶主要致香成分的种类分析,吹扫捕集-热脱附法适合分析低沸点醛类、酮类、烯烃类等易挥发性组分,同时蒸馏萃取法适合分析挥发性、半挥发性的酮类、醇类、酯类及高沸点难挥发酸、酯类组分,顶空固相微萃取法适合分析挥发性、半挥发性酮类、酯类、低碳分子酸等组分;从方法的重现性分析,进行6次平行试验,同时蒸馏萃取法所得致香成分TIC总峰面积RSD为3.94%,吹扫捕集-热脱附法为4.26%,顶空固相微萃取法为4.92%。

In order to compare the different preprocessing methods combined with gas chromatography-mass spectrometry(GC-MS) technology to analyze the differences of the type and content of aroma constituents in aging tobacco,adopt simultaneous distillation and extraction(SDE),headspace solid phase micro-extraction(HS-SPME),purge and trap-thermal desorption(P&T-TD) of these three pre-treatment methods for comparison.The results show that the method of P&T-TD detected a total of 129 kinds of aroma ingredients;the method of SDE detected 105 kinds of aroma compositions;the method of HS-SPME detected 101 kinds of aroma components.Analyzing from the types of the aroma constituents of tobacco,the method of P&T-TD was suitable for analysis of volatile components of low boiling point,such as aldehydes,ketones,olefins.The method of SDE was adapted to analyze volatile and semi-volatile ketones,alcohols,esters and the less volatile acids and esters of high boiling point.The method of HS-SPME was appropriate for analysis of volatile and semi-volatile ketones,esters,low carbon molecular acids;Analyzing from the reproducibility of the methods,conducting six times parallel experiments,TIC total peak area RSD of aroma ingredients by SDE was 3.94%;the method of P&T-TD was 4.26%;the method of HS-SPME was 4.92%.