摘要
用固相法合成了乙酰阿魏酰酪氨酰酪氨酸(F-Tyr-Tyr),并用ESI-MS、IR、NMR对其结构进行表征。应用紫外光谱法、荧光光谱法研究了F-Tyr-Tyr与小牛胸腺DNA(ctDNA)的相互作用。结果表明,F-Tyr-Tyr与ctDNA相互作用后体系的紫外吸收呈减色效应,且F-Tyr-Tyr加入后,DNA-EB体系的荧光强度减弱。根据Stern-Volmer方程求出F-Tyr-Tyr-DNA-EB体系的猝灭常数为K SV=6.28×103L·mol-1,猝灭方式为静态猝灭,推测F-Tyr-Tyr与ctDNA的相互作用模式主要是静电结合。
Aeetylferulic-Tyr-Tyr（ F-Tyr-Tyr ）was synthesized by solid phase method and characterized by ESI-MS,IR and NMR. The interaction between F-TyroTyr and ctDNA was investigated by ultraviolet spectroscopy and fluorescence spectroscopy. The results showed that the ultraviolet spectrum produced hypochromic effect and the fluorescence had quenching effect with increasing the concentration of F-Tyr-Tyr. According to the Stem-Volmer equation, the quenching constant was 6. 28× 10^3 L. mol^-1 and the quenching process was suggested as static. From these results,it can be predicted that the interaction between F-Tyr-Tyr and DNA was mainly in electrostatic binding mode.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2013年第9期1270-1274,共5页
Chemical Research and Application
基金
国家自然科学基金项目(20772023
21172054)资助
河南省创新型科技人才队伍建设工程项目(104200510022)资助
郑州市创新型科技人才队伍建设工程项目(10LJRC174)资助
关键词
阿魏酸酰胺衍生物
DNA
紫外光谱法
荧光光谱法
ferulic acid amide derivative
DNA
ultraviolet spectroscopy
fluorescence spectroscopy
