球孢白僵菌Bb080920菌株对番石榴实蝇的毒力测定

Toxicity Determination of Beauveria bassiana Bb080920 to Bactrocera correcta(Bezzi)

[目的]筛选出防治番石榴实蝇Beauveria bassiana(Bezzi)的有效生防菌。[方法]分别采用喷雾法和浸渍法测定不同浓度的球孢白僵菌Bb080920分生孢子液对番石榴实蝇成虫、幼虫和蛹的室内毒力。[结果]成虫的最大校正死亡率达(91.83±2.42)%,致死中浓度(LC50值)为(2.6281±0.2362)×108个/L,致死中时间(LT50值)为(4.61±0.37)d;幼虫最大累计校正死亡率达(95.23±3.13)%,LC50值为(2.5416±0.8395)×108个/L,LT50值为(4.36±0.42)d;蛹的累计校正死亡率最大达(82.59±2.78)%,LC50值为(2.7692±0.1538)×108个/L,LT50值为(5.05±0.68)d。[结论]球孢白僵菌Bb080920菌株毒力测定表明具有很高的毒性,未来有良好的应用前景。

[Aims] The paper aims to screen out effective biocontrol fungus against Bactrocera correcta （Bezzi）. [Methods] The virulence of Beauveria bassiana （Bb080920） conidia against different stages （adult, larvae and pupae） of 13. correcta （Bezzi） was tested in laboratory by spraying and dipping methods. [Results] The adjusted accumulative mortality rate of the adult was （91.83±2.42）%, the lethal concentration （LC50） was （2.628 1±0.2362）×10^8 spores/L, the lethal time （LT50） of adult was （4.61±0.37） d. The adjusted accumulative mortality rate of the larvae was （95.23±3.13）%, LC50 was （2.5416±0.8395）×10^8 spores/L, LT50 was （4.61±0.37） d. The adjusted accumulative mortality rate of the pupae was （82.59±2.78）%, LC50 was （2.7692±0.153 8）×10^8 spores/L, LT50 was （4.61±0.68） d. [Conclusions] These studies indicated that Beauveria bassiana Bb080920 has a high toxicity to B. correcta （Bezzi） with good prospect in the future.