β-细辛醚对抑郁模型大鼠行为及海马神经元的影响

Influences of β-asarone on rat behaviors and expressions of hippocampal neurons in rats with depression

目的 探讨β-细辛醚对抑郁模型大鼠行为学及海马Bcl-2、脑源性神经营养因子（BDNF）、酪氨酸激酶受体B（TrkB）、丝裂原活化蛋白激酶（MAPK）表达的影响.方法 60只2～3月龄SD大鼠随机分为正常对照组、模型组、氟西汀组和β-细辛醚组,每组15只.采用慢性轻度不可预见性应激加孤养复制抑郁模型,第2天开始,氟西汀组（1.2 mg/kg）和β-细辛醚组（25 mg/kg）灌胃给药,每日1次.于实验21 d,分别进行体重、糖水消耗量检测和敞箱实验,对大鼠行为学改变进行评定.采用免疫组化法检测Bcl-2、BDNF、TrkB、MAPK蛋白表达.结果 造模21d后,与正常对照组相比,模型组大鼠行为学指标明显降低（P＜0.05）;与模型组比较,氟西汀组和β-细辛醚组大鼠行为学指标明显改善（P＜0.05）;免疫组织化学检测结果显示,海马区Bcl-2、BDNF、TrkB、MAPK表达,模型组与正常对照组明显降低（P＜0.05）,氟西汀组和β-细辛醚组比模型组表达增强（P＜0.05）.结论 β-细辛醚可有效改善抑郁模型大鼠的抑郁状态,其机制可能与增加海马区Bcl-2、BDNF、TrkB、MAPK蛋白表达有关.

Objective To investigate the influences of β-asarone on rat behaviors and the expressions of hippocampal Bcl-2,BDNF,TrkB and MAPK in rats with depression.Methods SD rats （2-3 months old,n =60） were randomly divided into normal group,model group,fluoxetine group and β-asarone group （each =15）.The model of depression was established with chronic unpredictable stress and separation.The fluoxetine group and β-asarone group were orally given corresponding drugs once a day （fluoxetine：1.2 mg/kg and β-asarone：25 mg/kg） from the second day.The body weight and sucrose consumption were detected and open field test was carried out for assessing rat ethological changes on the 21st day.The protein expressions of Bcl-2,BDNF,TrkB and MAPK were detected by using immunohistochemistry technique.Results After modeling for 21 days,the ethological indexes decreased significantly in normal group compared with model group （P ＜ 0.05）.The ethological indexes were significantly improved in fluoxetine group and β-asarone group （P ＜ 0.05）.The results of immunohistochemistry technique showed that the expressions of Bcl-2,BDNF,TrkB and MAPK decreased significantly in model group and normal group （P ＜ 0.05）,and increased in fluoxetine group and β-asarone group （P ＜ 0.05）.Conclusion The depression of rat model can be relieved effectively by β-asarone,and the mechanism may be relatedt to the improvement of the expressions of Bcl-2,BDNF,TrkB and MAPK.