大黄丹参上调β-arrestin基因表达阻止大鼠实验性急性胰腺炎重症化进程

Rhubarb Salvia Increasing β-Arrestin Gene Expression to Prevent Rats from Experimental Acute Pancreatitis Intensive Process

目的:探讨生大黄和丹参通过上调β-arrestin基因表达,进而影响大鼠实验性急性胰腺炎重症化进程的作用机理。方法:建立SD大鼠实验性重症急性胰腺炎模型,分为正常组(SO)、实验对照组(SAP)、大黄丹参干预组(DD),检测脾脏组织中β-arrestin mRNA的表达、肝脏组织TRAF6蛋白含量水平、胰腺组织中NF-κBp65蛋白表达的水平。结果:SAP组β-arrestin mRNA表达受到显著抑制,DD组出现β-arrestin mRNA表达去阻抑现象;SAP组TRAF6蛋白含量水平呈下降变化,DD组TRAF6蛋白含量水平下降更明显;SAP组NF-κB转录活性增强,DD组NF-κB的活性受到抑制。结论:生大黄和丹参可以阻止急性胰腺炎的重症化进程,其机理与上调β-arrestin基因表达,弱化TLR-IL-1R信号系统的作用相关。

Objective ： To investigate the rhubarb and salvia increasing β- arrestin gene expression, thereby affecting the mechanism of the process of rats with experimental severe acute pancreatitis. Methods ： SD rats with experimental se- vere acute pancreatitis models were divided into normal group （SO） ,the experimental control group （SAP） ,rhubarb and salvia intervention group （DD）. β-arrestin mRNA expression of spleen tissue,liver tissue TRAF6 protein content lev- els, the level of NF - KB p65 protein expression in the pancreatic tissue were tested. Results ： The SAP group＇ s β-arres- tin mRNA expression was inhibited significantly. The DD group showed β- arrestin mRNA expression out of inhibition. TRAF6 protein content level downwardly changed in the SAP group. The DD group＇s TRAF6 protein content levels were decreased more significantly. The SAP group＇ s NF - KB transcription activity was enhanced While the DD group＇ s NF - KB activity was inhibited. Conclusion ： Rhubarb and salvia can block the process of severe acute pancreatitis. The mecha- nism may be related to increasing β- arrestin gene expression and weakening TLR - IL - 1R signaling system.