一测多评法优选关黄柏生物碱的超声提取工艺

Optimization of Ultrasonic Extraction Technology of Phellodendron amurense with Multi-components Quantitation by One Marker

目的:优选关黄柏生物碱的超声提取工艺。方法:采用一测多评法测定关黄柏中小檗碱、巴马汀和药根碱的含量,通过正交试验设计,以关黄柏中小檗碱、巴马汀和药根碱的含量之和为综合评价指标,优选关黄柏生物碱超声提取工艺。结果:最佳工艺条件为加10倍量70%乙醇超声提取2次,每次25 min。结论:优选的提取工艺稳定可行、操作简便、重复性好,可有效提取关黄柏中的多种生物碱。

Objective： To optimize ultrasonic extraction technology of Phellodendron amurense. Methods： Determined tThe contents of berberine, palmatine and jatrorrhizine were determined with multi - components quantitation by one marker,. The orthogonal design was used to optimize ultrasonic extraction technology of Phellodendron amurense with total contents of berberine, pahnatine and jatrorrhizine. Results： The best extracting condition was as follows ：70% alcohol ul- trasonic extracted 2 times, 25 minutes for everyeach time and ratio of lipuid to solid was 10 ： 1. Conclusion： The opti- mized technology was stable, simple, feasible and reproducible, and it was effective for extracting multiphasic alkaloids from Phellodendron amurense.