摘要
检测了抗迟缓爱德华菌单抗对牙鲆的保护性。从１０株自制单抗中筛选出一株对牙鲆具有较强保护性的单抗３Ｆ７。与仅以该菌感染的对照组相比，该抗体可显著提高牙鲆感染迟缓爱德华菌后的存活率。在以每条鱼０．１ｍＬ（１０９ＣＦＵ）菌量做攻击保护时，其保护率可达８０％。另外，利用所制单克隆抗体以免疫组织化学ＳＡＢＣ法检查了迟缓爱德华菌经腹腔感染牙鲆鱼后细菌的侵染途径，结果显示牙鲆对该菌易感的器官为肝、肾、牌等，而胃、肠等器官并不易被感染。
Protection of monoclone antibody against Edwardsiella tarda was evaluated in flounder. One mAb 3F7 was selected from 10 hybridoma cell lines, which could significantly enhance flounder resistance against E. tarda and prolonged their survival time compared with fish in control group administrated only with E. tarda. Protection rate of this mAb could reach 80% when flounder were challenged with E. tarda with dose of 0. 1mL (109 CFU). Furthermore, tissues of flounder died of E. tarda were detected following an immunohistochemical SABC method and the results suggested that liver, kidney, spleen were all easily infected by E. tarda, except for stomach and intestine.
出处
《水产学报》
CAS
CSCD
北大核心
2000年第6期554-559,共6页
Journal of Fisheries of China
基金
国家海洋"863"计划资助项目!(816-02-06)
关键词
迟缓爱德华菌
单克隆抗体
牙鲆
检测
防治
Edwardsiella tarda
monoclone antibody
flounder
detection
protection
