珠母贝糖胺聚糖的纯化及其化学性质

Purification and chemical properties of glycosaminoglycan extractea from Pinctada martensis

珠母贝糖胺聚糖粗制品经吸附、等电点沉淀、醇沉、溴化十六烷基三甲铵（ＣＴＡＢ）络合等法进行分离纯化，得一糖胺聚糖级分。经醋酸纤维素薄膜电泳显示为单一区带。化学组成分析表明：该级分氨基已糖含量较高（７１．３％），其中以氨基半乳精为主（５９％），含有少量的已糖醛酸，含硫酸基、半乳糖、岩藻糖。元素分析表明其碳、氢、氮、硫的含量分别为３７．７％、５．８％、５．８％、３．０％。对级分的残余氨基酸分析表明，８４．２％氨基酸已被清除。

A crude product of glycosaminoglycan (GAG) was prepared from whole viscera of Pinctada by the processes of enzymatic hydrolysis and enthanol precipitation and subsequently purified through carbon and diatomaceous earth adsorption and cetyltrimethylammonium bromide (CTAB) chelation. The actate film electrophoresis of purified GAG displayed a single band. The chemical components of purified GAG high content in hexosamine (71. 3% ) in winch galactosamine mainly accounts for 59% with less content of hexuronic acid, galactose, fucose and sulphonic acid group. In addition, the elemental analysis indicated the contents of carbon, hydrogen, nitrogen and sulfur being 37.7%, 5.8%, 5.8% and 3.0%, respectively. The total content of amino acids in purified GAG was 11. 3% showing that there was 84.2% of amino acids removed from dried whole viscera (71 .6% total amino acids).