两种香青中总黄酮含量测定及抗氧化活性

Determination of Total Flavones and Antioxidant Activity of Anaphalis lactea Maxim.And Anaphalis margaritacea Benth.

研究对乳白香青和珠光香青进行定性、定量比较分析,建立乳白香青与珠光香青总黄酮含量测定方法并进行对比分析。采用薄层层析实验对乳白香青与珠光香青中总黄酮进行定性鉴别;用紫外分光光度法,对两种香青的总黄酮含量及其抗氧化活性进行测定。经测定,乳白香青与珠光香青中总黄酮含量分别为40.43mg/mL和34.32mg/mL;乳白香青抑制DPPH自由基的能力IC50为2.96mg/mL;珠光香青I C50为3.488m g/mL。

To screen the extracting method of total flavones in Anaphalis lacteal Maxin and Anaphalis margaritacea Benth,this study took different methods to make a qualitative analysis,this research provide a scientific basis for further study of optimized extracting technique of total flavones. It also make full use of Anaphalis lacteal Maxin and Anaphalis margaritacea Benth resources. This study used rutin as a comparison,applied spectrophotometry at 500 nm to determine the content of total flavones in. The qualitative analysis is mainly in chemical analysis ways. By comparing and analysis,The content of total flavones in Anaphalis lacteal Maxin （40. 43 mg/mL） relatively higher than in Anaphalis margaritacea Benth （34.32 mg/mL）.