比色法测定Annexin V变体冻干品中的氯化亚锡含量

Colorimetric Determination of Stannous Chloride in Annexin V Derivative Kit

测定自制的A nnex in V变体冻干品中的氯化亚锡含量。在酸性条件下,用钼酸钠-硫氰酸钾比色法测定,K=460nm。亚锡在5—50Lg内线性关系良好,低、中、高三浓度平均回收率分别为102.9%,99.9%,101.0%;RSD分别为6.4%,8.1%,5.1%(n=6)。用比色法测定A nnex in V变体冻干品中亚锡含量,方法简便、迅捷、试剂易得,可用于冻干品中氯化亚锡的质量控制。

Content of stannous chloride in annexin V derivative kit made by ourselves was determined. Sn（Ⅱ） is determinated by colorimetry with sodium molybdate and potassium thiocyanate in acid condition,The wave length was 460nm. The linearity of Sn（ Ⅱ ） was favorable in the range of 5--50μg. The Low, medium, and high average recovery was 102. 9%, 99. 9%, 101. 0% and the relative standard deviation was 6. 4%, 8. 1%, 5. 1% respectively. Colorimetric determination of stannous chloride is easily, and the result is satisfactory. It can be applied for quality control of stannous chloride in kit.