索氏提取法提取豆腐柴根茎叶中的总黄酮

Extraction of Total Flavonoids from Roots,Stems and Leaves ofPremna microphyllaTurcz by Soxhlet Methods

用索氏提取法提取豆腐柴根、茎、叶中总黄酮并进行含量测定。以芦丁为标准品,用分光光度法在502nm处测定提取物中总黄酮的含量。考察乙醇浓度、料液比和时间对于提取效率的影响。结果表明,60%的乙醇浓度、1∶40g/mL的料液比和提取时间为2.0h是提取豆腐柴中黄酮化合物的最优条件。在此条件下,根总黄酮含量为5.034mg/g、茎为5.785mg/g、叶为23.148mg/g。

The contents of total flavonoids were extracted from roots,stems and leaves of Premna microphylla Turcz by Soxhlet methods,that were determined by spectrophotometry at 502 nm with the rutin as reference substance. The effects of different ethanol elution concentration,solid-to-liquid ratio and extraction time on the extraction rate of total flavonoids were investigated. The results 23. 148mg/g.