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胰腺星状细胞在2型糖尿病模型大鼠胰岛纤维化的作用 被引量：2

The role of pancreatic stellate cells in islet fibrosis of T2DM rat models
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摘要 目的观察胰腺星状细胞(PSC)在T2DM模型GK大鼠胰岛中的存在状态,探讨PSC在胰岛纤维化发生发展中的作用。方法GK与Wistar大鼠胰腺冰冻切片行Masson染色和荧光双标(α-SMA/Insulin)染色。采用外向生长法分离纯化GK大鼠胰岛外生细胞,免疫荧光检测Vimentin,α-平滑肌肌动蛋白(α-SMA),Fibronectin(FN)和ColⅠ/Ⅲ的表达。结果 GK大鼠胰岛纤维化区域α-SMA染色阳性细胞增多,胰岛素分泌缺失。大量PSC自GK大鼠胰岛外生,表达Vimentin、α-SMA、FN和ColⅠ/Ⅲ。结论活化PSC存在于T2DM模型GK大鼠纤维化胰岛中,并在T2DM胰岛纤维化中发挥了一定的作用。 Objective To observe pancreatic stellate cell （PSC） existing in GK rat islets and explore the role of SC in the progression of islet fibrosis. Methods Masson staining and immunofluorescence staining assay （α-SMA/insulin） were performed using frozen sections of GK/Wistar rat pancreas. Isolating PSC by outgrowth method, and immunofluorescence staining assay for α-SMA, Vimentin, Fibronectin and Col type Ⅰ/Ⅲ were performed. Results The α-SMA positive cells increased and beta cell mass decreased in the fibrotic area in GK rat islet. Large number of SC grew out from GK rat islets, and these cells expressed α-SMA,Vimentin,FN and Col Ⅰ/Ⅲ. Conclusion The activated PSC present in fibrotic islets of GK rats and they may play an role in T2DM islet fibrosis.
作者 李凤飞 孙子林 陈碧君 殷俊梅 翟清 查敏
机构地区 东南大学附属中大医院内分泌科 东南大学医学院糖尿病研究所
出处 《中国糖尿病杂志》 CAS CSCD 北大核心 2013年第9期828-831,共4页 Chinese Journal of Diabetes
基金 国家自然基金面上项目(30971399 81170716 81270010)
关键词 糖尿病 2型 胰腺星状细胞 胰岛纤维化 GK大鼠 Dabetes mellitus,type 2 Pancreatic stellate cells Islet fibrosis Goto-Kakizaki rats
分类号 R576 [医药卫生—消化系统]
  • 相关文献

参考文献15

  • 1Tarry-Adkins JL,Chen JH,Jones RH. Poor maternal nutrition leads to alterations in oxidative stress,antioxidant defense capacity,and markers of fibrosis in rat islets:potential underlying mechanisms for development of the diabetic phenotype in later life[J].{H}FASEB JOURNAL,2010.2762-2771.
  • 2Homo-Delarche F,Calderari S,Irminger JC. Islet in ammation and fibrosis in a spontaneous model of type 2 diabetes,the GK rat[J].{H}DIABETES,2006.1625-1633.
  • 3Habibi J,Whaley-Connell A,Hayden MR. Renin inhibition attenuates insulin resistance,oxidative stress,and pancreatic remodeling in the transgenic Ren2 rat[J].{H}ENDOCRINOLOGY,2008.5643-5653.
  • 4Fuse M,Yokoi N,Shinohara M. Identification of a major locus for islet inflammation and fibrosis in the spontaneouslydiabetic Torii rat[J].{H}Physiological Genomics,2008.96-105.
  • 5Mizukami H,Wada R,Yonezawa A. Suppression of postprandial hyperglycaemia by pioglitazone improved islet fibrosis and macrophage migration in the Goto-Kakizaki rat[J].{H}Diabetes Obesity & Metabolism,2008.791-794.
  • 6Tikellis C,Wookey PJ,Candido R. Improved islet morphology after blockade of the renin-angiotensin system in the ZDF rat[J].{H}DIABETES,2004.989-997.
  • 7Zhao HL,Lai FM,Tong PC. Prevalence and clinicopathological characteristics of islet amyloid in Chinese patients with type 2 diabetes[J].{H}DIABETES,2003.2759-2766.
  • 8Hong OK,Lee SH,Rhee M. Hyperglycemia and hyperinsulinemia have additive effects on activation and proliferation of pancreatic stellate cells:possible explanation of islet-specific fibrosis in type 2 diabetes mellitus[J].{H}Journal of Cellular Biochemistry,2007.665-675.
  • 9潘琳,李宏亮,杨文英,杜瑞琴,王冰,楼大钧,肖建中,李光伟.胰腺星状细胞活化在高脂饲养大鼠胰岛纤维化形成中的作用[J].中华内分泌代谢杂志,2009,25(1):25-27. 被引量：2
  • 10Masuyama T,Komeda K,Hara A. Chronological characterization of diabetes development in male Spontaneously Diabetic Torii rats[J].Bi0chem Biophys Res Commun,2004.870-877.

二级参考文献42

  • 1邬云红,李秀钧,李宏亮,王煜,张玄娥,柯柳,张祥迅.高脂饮食肥胖大鼠胰岛细胞胰岛素抵抗机理的探讨[J].中华医学杂志,2005,85(27):1907-1910. 被引量：56
  • 2王昕,杨文英,萧建中,赵文惠,王娜,刘雪丽,潘琳.高脂饲养及罗格列酮干预对α细胞功能的影响[J].中华内科杂志,2005,44(8):601-605. 被引量：16
  • 3Ko SH, Kwon HS, Kim SR, et al. Ramipril treatment suppresses islet fibrosis in Otsuka Long-Evans Tokushima fatty rats. Biochem Biophys Res Commun, 2004,316 : 114-122.
  • 4Hong OK, Lee SH, Rhee M, et al. Hyperglycemia and hyper- insulinemia have additive effects on activation and proliferation of pancreatic stellate cells : possible explanation of islet specific fibrosis in type 2 diabetes mellitus. J Cell Biochem, 2007,101:665-675.
  • 5Nomiyama Y, Tashiro M, Yamaguchi T, et al. High glucose activates rat pancreatic stellate is through protein kinase C and p38 mitogen-activated protein kinase pathway. Pancreas, 2007,34 : 364-372.
  • 6Phillips PA, Wu M J, Kumar RK, et al. Cell migration: a novel aspect of pancreatic stellate cell biology. Gut, 2003,52:677-682.
  • 7Longnecker DS. Pathology and pathogenesis of disease of the pancreas. Am J Pathol, 1982,107 : 103-121.
  • 8Jia D, Yamamoto M, Otani M, et al. Bezafibrate on lipids and glucose metabolism in obese diabetic Otsuka Long-Evans Tokushima fatty rats. Metabolism, 2004,53:405-413.
  • 9Yuan M, Konstantopoulos N, Lee JS, et al. Reversal of obesity- and diet-induced insulin resistance with salicylates or targeted disruption of IKKβ. Science, 2001,293:1673-1677.
  • 10Zhou YP,Madjidi A,Wilson ME,et al.Matrix metalloproteinases contribute to insulin insufficiency in zucker diabetic fatty rats.Diabetes,2005,54:2612-2619.

共引文献4

同被引文献12

  • 1朱波,薛耀明,李晨钟,潘永华,张燕.自发性2型糖尿病大鼠病程进展与胰腺形态学改变[J].实用医学杂志,2005,21(16):1742-1744. 被引量：2
  • 2Xu Yu, Wang Limin, He Jiang, et al. Prevalence and Control of Diabetes in Chinese Adults[J]. JAMA, 2013, 310(9): 948 -959.
  • 3Tikellis C, Wookey P J, Candido R, et al. Improved islet morphology after blockade of the renin-angiotensin system in the ZDF rat[J]. Diabetes, 2004, 53 (4) : 989 - 997.
  • 4Bachena M G, Schneider E, Gross H, et al. Identification, culture, and characterization of pancreatic stellate cells in rats and humans[ J]. Gastroenterology, 1998, 115(2) : 421 -432.
  • 5McCarroll J A, Phillips P A, Santucci N, et al. Vitamin A inhibits pancreatic stellate cell actlvation: implications for treatment of pancreatic fibrosis [ J]. Gut, 2006, 55 ( 1 ) : 79 - 89.
  • 6Homo D F, Calderari S, Irminger J C, et al. Islet inflammation and fibrosis in a spontaneous model of type 2 diabetes, the GK rat [J]. Diabetes, 2006; 55(6): 1625 -3163.
  • 7Giroix M H, Vesco L, Portha B. Functional andmetabolic perturbations in isolated pancreatic islets from the GK rat, a genetic model of noninsulin-dependent diabetes [ J ]. Endocrinology, 1993, 132(2) : 815 -822.
  • 8Bachem M G, Schneider E, Gross H, et al. Identification, culture, and characterization of pancreatic stellate cells in rats and humans[J]. Gastroenterology, 1998, 115(2):421 -432.
  • 9McCarroll J A, Phillips P A, Santucci N, et al. Vitamin A inhibits pancreatic stellate cell activation: implications for treatment of pancreatic fibrosis [ J]. Gut, 2006, 55 ( 1 ) : 79 -89.
  • 10田小芸,董敏,赵志刚,许龙祥,刘彪,郭联庆,恽时锋.自发性糖尿病小鼠C57BL/KsJ-db/db某些脏器组织病理学观察[J].实验动物科学,2011,28(1):18-20. 被引量：7

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中国糖尿病杂志
2013年 第9期

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